Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10300 and 10301 are separated by 435 nucleotides10301 and 10302 are separated by 500 nucleotides10302 and 10303 are separated by 267 nucleotides10303 and 10304 are separated by 341 nucleotides mRNA_1932: 10300 - BLAST family 69 glycosyltransferase [Melampsora larici-..., at 544,727 to 545,015 10300 mRNA_1933: 10301 - Hypothetical Protein, at 545,451 to 545,690 10301 mRNA_1934: 10302 - HMMPfam-Cryptococcal mannosyltransferase 1-PF11735, at 546,191 to 548,410 10302 mRNA_1935: 10303 - K00654 SPT serine palmitoyltransferase, at 548,678 to 550,878 10303 mRNA_1936: 10304 - K07424 CYP3A cytochrome P450, family 3, subfamily A, at 551,220 to 553,834 10304
Group Condition mRNA_1932 mRNA_1933 mRNA_1934 mRNA_1935 mRNA_1936
10300 10301 10302 10303 10304
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 -0.2 -3.5 N.D. -0.6
ypd buoyancy 40 hrs No Buoyancy Separation -0.6 -0.8 -0.6 N.D. +1.0
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 +0.6 -1.1 N.D. -0.3
ypd buoyancy 40 hrs Median Buoyancy +0.4 -0.2 -0.9 N.D. -0.0
ypd buoyancy 40 hrs High Buoyancy +0.3 -0.5 -0.6 N.D. -0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.0 +0.4 -1.2 N.D. +0.2
growth on oleic acid YNB Oleic Acid -0.5 +0.0 +0.6 N.D. -0.0
facs on lipid accumulation Sorted No BODIPY Gate +0.5 +0.1 -0.1 N.D. +0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.7 -0.4 -0.2 N.D. +0.5
growth on oleic acid YNB Glucose -0.3 -0.1 +1.1 N.D. +0.1
growth on ricinoleic acid YNB Glucose -0.6 +0.2 +1.4 N.D. +0.1
ypd buoyancy 40 hrs Low Buoyancy +0.3 -0.3 +1.5 N.D. +0.0
auxotrophy YNB Glucose 75mM Methionine +0.2 +0.4 +0.8 N.D. +0.2
auxotrophy YNB Glucose 75mM Arginine +0.3 +0.5 +0.9 N.D. +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.6 +0.2 +0.8 N.D. +0.4
auxotrophy YNB Glucose +0.4 +0.6 +0.8 N.D. +0.1
auxotrophy Dropout Complete +0.7 +0.6 +1.2 N.D. -0.4
growth on ricinoleic acid YNB Methylricinoleic Acid -0.7 +0.4 +3.0 N.D. -0.0
growth on ricinoleic acid YNB Ricinoleic Acid -0.7 +0.2 +3.1 N.D. +0.2
facs on lipid accumulation Sorted High BODIPY Gate +0.1 +0.2 +2.4 N.D. +0.2
growth on ypd YPD +3.7 +0.2 +0.3 N.D. -0.3
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