Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10302 and 10303 are separated by 267 nucleotides10303 and 10304 are separated by 341 nucleotides10304 and 10305 are separated by 559 nucleotides10305 and 10306 are separated by 551 nucleotides mRNA_1934: 10302 - HMMPfam-Cryptococcal mannosyltransferase 1-PF11735, at 546,191 to 548,410 10302 mRNA_1935: 10303 - K00654 SPT serine palmitoyltransferase, at 548,678 to 550,878 10303 mRNA_1936: 10304 - K07424 CYP3A cytochrome P450, family 3, subfamily A, at 551,220 to 553,834 10304 mRNA_1937: 10305 - K18342 OTUD6 OTU domain-containing protein 6, at 554,394 to 556,031 10305 mRNA_1938: 10306 - HMMPfam-Amidohydrolase family-PF07969,SUPERFAMILY--SSF51338,SUPERFAMILY--SSF51556, at 556,583 to 558,262 10306
Group Condition mRNA_1934 mRNA_1935 mRNA_1936 mRNA_1937 mRNA_1938
10302 10303 10304 10305 10306
facs on lipid accumulation Sorted Low BODIPY Gate -3.5 N.D. -0.6 -2.0 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -1.1 N.D. -0.3 -1.2 -0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.1 N.D. +0.0 -2.2 -0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy -1.2 N.D. +0.2 -0.3 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 N.D. +0.5 -1.3 -0.0
ypd buoyancy 40 hrs Median Buoyancy -0.9 N.D. -0.0 -0.2 +0.2
ypd buoyancy 40 hrs High Buoyancy -0.6 N.D. -0.0 +0.0 -0.1
growth on oleic acid YNB Oleic Acid +0.6 N.D. -0.0 -1.0 -0.2
growth on ypd YPD +0.3 N.D. -0.3 -0.3 -0.1
facs on lipid accumulation Sorted High BODIPY Gate +2.4 N.D. +0.2 -2.5 -0.3
auxotrophy Dropout Complete +1.2 N.D. -0.4 -0.8 -0.2
auxotrophy YNB Glucose +0.8 N.D. +0.1 -0.9 +0.2
auxotrophy YNB Glucose 75mM Methionine +0.8 N.D. +0.2 -0.9 +0.3
growth on oleic acid YNB Glucose +1.1 N.D. +0.1 -0.7 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation -0.6 N.D. +1.0 +0.7 -0.3
auxotrophy YNB Glucose 75mM Arginine +0.9 N.D. +0.1 -0.2 +0.2
growth on ricinoleic acid YNB Glucose +1.4 N.D. +0.1 -0.9 +0.7
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.8 N.D. +0.4 +0.3 -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid +3.0 N.D. -0.0 -1.8 +0.6
growth on ricinoleic acid YNB Ricinoleic Acid +3.1 N.D. +0.2 -1.9 +0.6
ypd buoyancy 40 hrs Low Buoyancy +1.5 N.D. +0.0 +0.4 +0.1
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