Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10303 and 10304 are separated by 341 nucleotides10304 and 10305 are separated by 559 nucleotides10305 and 10306 are separated by 551 nucleotides10306 and 10307 are separated by 317 nucleotides mRNA_1935: 10303 - K00654 SPT serine palmitoyltransferase, at 548,678 to 550,878 10303 mRNA_1936: 10304 - K07424 CYP3A cytochrome P450, family 3, subfamily A, at 551,220 to 553,834 10304 mRNA_1937: 10305 - K18342 OTUD6 OTU domain-containing protein 6, at 554,394 to 556,031 10305 mRNA_1938: 10306 - HMMPfam-Amidohydrolase family-PF07969,SUPERFAMILY--SSF51338,SUPERFAMILY--SSF51556, at 556,583 to 558,262 10306 mRNA_1939: 10307 - KOG0255 Synaptic vesicle transporter SVOP and related transporters (major facilitator superfamily), at 558,580 to 560,603 10307
Group Condition mRNA_1935 mRNA_1936 mRNA_1937 mRNA_1938 mRNA_1939
10303 10304 10305 10306 10307
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.2 -2.5 -0.3 +0.3
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.6 -2.0 -0.1 +0.4
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.0 -2.2 -0.4 +0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.3 -1.2 -0.1 -0.3
auxotrophy Dropout Complete N.D. -0.4 -0.8 -0.2 +0.0
growth on oleic acid YNB Oleic Acid N.D. -0.0 -1.0 -0.2 -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.0 -1.8 +0.6 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.5 -1.3 -0.0 -0.2
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.2 -1.9 +0.6 +0.1
growth on ypd YPD N.D. -0.3 -0.3 -0.1 -0.3
auxotrophy YNB Glucose 75mM Methionine N.D. +0.2 -0.9 +0.3 -0.2
growth on oleic acid YNB Glucose N.D. +0.1 -0.7 +0.2 -0.1
ypd buoyancy 40 hrs High Buoyancy N.D. -0.0 +0.0 -0.1 -0.4
auxotrophy YNB Glucose N.D. +0.1 -0.9 +0.2 +0.1
growth on ricinoleic acid YNB Glucose N.D. +0.1 -0.9 +0.7 -0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.0 -0.2 +0.2 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.2 -0.3 +0.1 -0.1
auxotrophy YNB Glucose 75mM Arginine N.D. +0.1 -0.2 +0.2 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.4 +0.3 -0.0 -0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.0 +0.4 +0.1 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +1.0 +0.7 -0.3 -0.2
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