Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10308 and 10309 are separated by 429 nucleotides10309 and 10310 are separated by 432 nucleotides10310 and 10311 are separated by 240 nucleotides10311 and 10312 are separated by 390 nucleotides mRNA_1940: 10308 - K00276 AOC3, AOC2, tynA primary-amine oxidase, at 561,164 to 563,777 10308 mRNA_1941: 10309 - KOG3798 Predicted Zn-dependent hydrolase (beta-lactamase superfamily), at 564,207 to 567,007 10309 mRNA_1942: 10310 - HMMPfam-PLD-like domain-PF13091,ProSiteProfiles-Phospholipase D phosphodiesterase active site profile.-PS50035,SMART-Phospholipase D. Active site motifs.-SM00155,SUPERFAMILY--SSF56024, at 567,440 to 570,897 10310 mRNA_1943: 10311 - Hypothetical Protein, at 571,138 to 572,203 10311 mRNA_1944: 10312 - BLAST Respiratory supercomplex factor 2, mitochondrial [Cla..., at 572,594 to 573,767 10312
Group Condition mRNA_1940 mRNA_1941 mRNA_1942 mRNA_1943 mRNA_1944
10308 10309 10310 10311 10312
growth on ypd YPD -0.2 -0.1 -0.5 -0.2 -0.4
ypd buoyancy 40 hrs No Buoyancy Separation +0.5 -0.2 -1.0 +0.6 -1.2
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 -0.4 -0.6 -0.2 -0.5
growth on ricinoleic acid YNB Methylricinoleic Acid +0.3 -0.3 -0.6 -0.2 -0.2
auxotrophy YNB Glucose 75mM Methionine +0.1 -0.5 -0.2 -0.1 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.5 +0.5 -0.6 -0.0 -0.3
growth on oleic acid YNB Oleic Acid -0.0 -0.2 -0.5 -0.2 +0.1
auxotrophy YNB Glucose 75mM Arginine +0.1 -0.2 -0.3 -0.3 +0.0
auxotrophy YNB Glucose +0.0 +0.1 +0.1 -0.5 -0.3
facs on lipid accumulation Sorted No BODIPY Gate -0.1 -0.3 -0.3 -0.0 +0.3
auxotrophy Dropout Complete +0.1 -0.1 -0.1 -0.1 -0.2
facs on lipid accumulation Sorted Low BODIPY Gate +0.0 -0.1 -0.8 -0.0 +0.6
growth on ricinoleic acid YNB Glucose +0.3 +0.4 -0.3 -0.4 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 -0.7 +0.7 +0.1 -0.2
growth on oleic acid YNB Glucose -0.0 -0.3 +0.1 -0.1 +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 +0.2 -0.1 -0.2 +0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.1 +0.5 -0.4 -0.0 +0.1
ypd buoyancy 40 hrs Low Buoyancy -0.2 +0.4 -0.0 +0.1 +0.2
ypd buoyancy 40 hrs Median Buoyancy -0.2 +0.0 -0.1 +0.3 +0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 +0.4 -0.0 +0.2 +0.1
ypd buoyancy 40 hrs High Buoyancy +0.1 +0.3 -0.2 +0.3 +0.3
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