Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10310 and 10311 are separated by 240 nucleotides10311 and 10312 are separated by 390 nucleotides10312 and 10313 are separated by 583 nucleotides10313 and 10314 overlap by 25 nucleotides mRNA_1942: 10310 - HMMPfam-PLD-like domain-PF13091,ProSiteProfiles-Phospholipase D phosphodiesterase active site profile.-PS50035,SMART-Phospholipase D. Active site motifs.-SM00155,SUPERFAMILY--SSF56024, at 567,440 to 570,897 10310 mRNA_1943: 10311 - Hypothetical Protein, at 571,138 to 572,203 10311 mRNA_1944: 10312 - BLAST Respiratory supercomplex factor 2, mitochondrial [Cla..., at 572,594 to 573,767 10312 mRNA_1945: 10313 - Hypothetical Protein, at 574,351 to 575,148 10313 mRNA_1946: 10314 - KOG2458 Endoplasmic reticulum protein EP58, contains filamin rod domain and KDEL motif, at 575,124 to 577,080 10314
Group Condition mRNA_1942 mRNA_1943 mRNA_1944 mRNA_1945 mRNA_1946
10310 10311 10312 10313 10314
facs on lipid accumulation Sorted Low BODIPY Gate -0.8 -0.0 +0.6 -3.0 -4.7
ypd buoyancy 40 hrs No Buoyancy Separation -1.0 +0.6 -1.2 -1.1 -1.6
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.6 -0.0 -0.3 -1.2 -2.1
facs on lipid accumulation Sorted No BODIPY Gate -0.3 -0.0 +0.3 -1.3 -1.8
ypd buoyancy 40 hrs High Buoyancy -0.2 +0.3 +0.3 -0.5 -1.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.0 +0.2 +0.1 -0.3 -0.9
growth on ypd YPD -0.5 -0.2 -0.4 +0.2 +0.5
ypd buoyancy 40 hrs Median Buoyancy -0.1 +0.3 +0.4 -0.4 -0.7
auxotrophy YNB Glucose +0.1 -0.5 -0.3 +0.3 +0.4
auxotrophy YNB Glucose 75mM Methionine -0.2 -0.1 -0.2 +0.3 +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 -0.2 +0.1 +0.6 -0.2
auxotrophy YNB Glucose 75mM Arginine -0.3 -0.3 +0.0 +0.4 +0.5
growth on ricinoleic acid YNB Glucose -0.3 -0.4 -0.2 +0.8 +0.5
growth on oleic acid YNB Oleic Acid -0.5 -0.2 +0.1 +0.6 +0.7
facs on lipid accumulation Sorted High BODIPY Gate -0.4 -0.0 +0.1 +0.6 +0.7
auxotrophy Dropout Complete -0.1 -0.1 -0.2 +0.5 +1.0
growth on ricinoleic acid YNB Methylricinoleic Acid -0.6 -0.2 -0.2 +1.7 +0.9
growth on oleic acid YNB Glucose +0.1 -0.1 +0.3 +0.6 +1.0
growth on ricinoleic acid YNB Ricinoleic Acid -0.6 -0.2 -0.5 +1.4 +1.8
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.7 +0.1 -0.2 +1.0 +0.4
ypd buoyancy 40 hrs Low Buoyancy -0.0 +0.1 +0.2 +1.6 +1.2
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