Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10314 and 10315 are separated by 264 nucleotides10315 and 10316 are separated by 1724 nucleotides10316 and 10317 are separated by 491 nucleotides10317 and 10318 are separated by 1235 nucleotides mRNA_1946: 10314 - KOG2458 Endoplasmic reticulum protein EP58, contains filamin rod domain and KDEL motif, at 575,124 to 577,080 10314 mRNA_1947: 10315 - K14864 FTSJ1, TRM7 tRNA (cytidine32/guanosine34-2'-O)-methyltransferase, at 577,345 to 578,684 10315 mRNA_1948: 10316 - K08176 PHO84 MFS transporter, PHS family, inorganic phosphate transporter, at 580,409 to 582,858 10316 mRNA_1949: 10317 - KOG0254 Predicted transporter (major facilitator superfamily), at 583,350 to 585,589 10317 mRNA_1950: 10318 - K03965 NDUFB9 NADH dehydrogenase (ubiquinone) 1 beta subcomplex subunit 9, at 586,825 to 587,644 10318
Group Condition mRNA_1946 mRNA_1947 mRNA_1948 mRNA_1949 mRNA_1950
10314 10315 10316 10317 10318
facs on lipid accumulation Sorted Low BODIPY Gate -4.7 +0.4 -0.2 -0.4 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -2.1 -0.5 +0.1 -0.1 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -1.6 -1.2 +0.2 +0.2 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -1.8 +0.3 +0.1 -0.4 N.D.
ypd buoyancy 40 hrs High Buoyancy -1.1 -0.5 -0.1 +0.1 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 -0.4 -0.1 -0.0 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.9 +0.1 +0.2 +0.1 N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.7 +0.4 -0.0 -0.1 N.D.
growth on ypd YPD +0.5 -0.8 +0.1 -0.1 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.4 +0.1 -0.0 -0.6 N.D.
growth on ricinoleic acid YNB Glucose +0.5 +0.0 -0.1 -0.3 N.D.
growth on oleic acid YNB Glucose +1.0 -0.3 -0.0 +0.1 N.D.
auxotrophy YNB Glucose +0.4 +0.1 +0.2 +0.1 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.3 +0.0 +0.1 +0.4 N.D.
growth on oleic acid YNB Oleic Acid +0.7 -0.1 +0.1 +0.2 N.D.
auxotrophy Dropout Complete +1.0 -0.1 -0.0 +0.2 N.D.
facs on lipid accumulation Sorted High BODIPY Gate +0.7 +0.3 +0.1 -0.0 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.9 +0.1 -0.1 +0.2 N.D.
ypd buoyancy 40 hrs Low Buoyancy +1.2 +0.3 -0.0 -0.0 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.5 +0.3 +0.4 +0.3 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +1.8 -0.2 +0.0 +0.2 N.D.
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