Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10318 and 10319 are separated by 506 nucleotides10319 and 10320 are separated by 377 nucleotides10320 and 10321 are separated by 507 nucleotides10321 and 10322 are separated by 115 nucleotides mRNA_1950: 10318 - K03965 NDUFB9 NADH dehydrogenase (ubiquinone) 1 beta subcomplex subunit 9, at 586,825 to 587,644 10318 mRNA_1951: 10319 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF57701, at 588,151 to 591,426 10319 mRNA_1952: 10320 - K14564 NOP56 nucleolar protein 56, at 591,804 to 593,975 10320 mRNA_1953: 10321 - K18442 ARFGEF, BIG brefeldin A-inhibited guanine nucleotide-exchange protein, at 594,483 to 601,309 10321 mRNA_1954: 10322 - Hypothetical Protein, at 601,425 to 602,462 10322
Group Condition mRNA_1950 mRNA_1951 mRNA_1952 mRNA_1953 mRNA_1954
10318 10319 10320 10321 10322
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.9 N.D. N.D. -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.8 N.D. N.D. -0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.7 N.D. N.D. +0.1
growth on ricinoleic acid YNB Glucose N.D. -0.4 N.D. N.D. -0.2
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.5 N.D. N.D. +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.5 N.D. N.D. +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.1 N.D. N.D. -0.2
growth on oleic acid YNB Glucose N.D. -0.1 N.D. N.D. -0.1
ypd buoyancy 40 hrs High Buoyancy N.D. -0.1 N.D. N.D. -0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.3 N.D. N.D. +0.3
growth on oleic acid YNB Oleic Acid N.D. +0.1 N.D. N.D. -0.2
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.1 N.D. N.D. -0.2
auxotrophy YNB Glucose 75mM Arginine N.D. +0.1 N.D. N.D. -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.0 N.D. N.D. -0.0
auxotrophy YNB Glucose 75mM Methionine N.D. +0.2 N.D. N.D. -0.2
auxotrophy YNB Glucose N.D. +0.1 N.D. N.D. -0.0
growth on ypd YPD N.D. -0.4 N.D. N.D. +0.5
auxotrophy Dropout Complete N.D. +0.1 N.D. N.D. +0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.3 N.D. N.D. +0.0
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.2 N.D. N.D. +0.2
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.5 N.D. N.D. +0.3
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