Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10319 and 10320 are separated by 377 nucleotides10320 and 10321 are separated by 507 nucleotides10321 and 10322 are separated by 115 nucleotides10322 and 10323 are separated by 998 nucleotides mRNA_1951: 10319 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF57701, at 588,151 to 591,426 10319 mRNA_1952: 10320 - K14564 NOP56 nucleolar protein 56, at 591,804 to 593,975 10320 mRNA_1953: 10321 - K18442 ARFGEF, BIG brefeldin A-inhibited guanine nucleotide-exchange protein, at 594,483 to 601,309 10321 mRNA_1954: 10322 - Hypothetical Protein, at 601,425 to 602,462 10322 mRNA_1955: 10323 - KOG0379 Kelch repeat-containing proteins, at 603,461 to 605,817 10323
Group Condition mRNA_1951 mRNA_1952 mRNA_1953 mRNA_1954 mRNA_1955
10319 10320 10321 10322 10323
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.8 N.D. N.D. -0.1 -0.8
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.9 N.D. N.D. -0.2 -0.5
growth on ricinoleic acid YNB Glucose -0.4 N.D. N.D. -0.2 +0.2
growth on oleic acid YNB Glucose -0.1 N.D. N.D. -0.1 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.7 N.D. N.D. +0.1 +0.2
growth on oleic acid YNB Oleic Acid +0.1 N.D. N.D. -0.2 -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.5 N.D. N.D. +0.1 +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 N.D. N.D. -0.2 -0.0
ypd buoyancy 40 hrs High Buoyancy -0.1 N.D. N.D. -0.1 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate -0.5 N.D. N.D. +0.2 +0.3
facs on lipid accumulation Sorted High BODIPY Gate -0.3 N.D. N.D. +0.3 +0.1
auxotrophy Dropout Complete +0.1 N.D. N.D. +0.1 -0.1
auxotrophy YNB Glucose 75mM Methionine +0.2 N.D. N.D. -0.2 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.0 N.D. N.D. -0.0 +0.2
auxotrophy YNB Glucose 75mM Arginine +0.1 N.D. N.D. -0.2 +0.2
auxotrophy YNB Glucose +0.1 N.D. N.D. -0.0 +0.2
facs on lipid accumulation Sorted No BODIPY Gate +0.1 N.D. N.D. -0.2 +0.3
ypd buoyancy 40 hrs Low Buoyancy +0.3 N.D. N.D. +0.0 -0.1
ypd buoyancy 40 hrs Median Buoyancy +0.2 N.D. N.D. +0.2 -0.1
growth on ypd YPD -0.4 N.D. N.D. +0.5 +0.3
ypd buoyancy 40 hrs No Buoyancy Separation +0.5 N.D. N.D. +0.3 +0.0
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