Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10323 and 10324 are separated by 1153 nucleotides10324 and 10325 are separated by 366 nucleotides10325 and 10326 are separated by 722 nucleotides10326 and 10327 are separated by 675 nucleotides mRNA_1955: 10323 - KOG0379 Kelch repeat-containing proteins, at 603,461 to 605,817 10323 mRNA_1956: 10324 - BLAST 40S ribosomal protein S1 [Exophiala sideris], at 606,971 to 607,614 10324 mRNA_1957: 10325 - K02984 RP-S3Ae, RPS3A small subunit ribosomal protein S3Ae, at 607,981 to 609,583 10325 mRNA_1958: 10326 - KOG0255 Synaptic vesicle transporter SVOP and related transporters (major facilitator superfamily), at 610,306 to 612,524 10326 mRNA_1959: 10327 - Hypothetical Protein, at 613,200 to 613,373 10327
Group Condition mRNA_1955 mRNA_1956 mRNA_1957 mRNA_1958 mRNA_1959
10323 10324 10325 10326 10327
facs on lipid accumulation Sorted High BODIPY Gate +0.1 -1.0 N.D. -0.2 N.D.
growth on oleic acid YNB Glucose -0.1 -0.1 N.D. -0.4 N.D.
auxotrophy Dropout Complete -0.1 -0.1 N.D. -0.4 N.D.
growth on oleic acid YNB Oleic Acid -0.2 -0.0 N.D. -0.3 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.8 +0.5 N.D. -0.2 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.0 -0.3 N.D. -0.2 N.D.
ypd buoyancy 40 hrs High Buoyancy -0.1 -0.5 N.D. +0.2 N.D.
auxotrophy YNB Glucose +0.2 -0.1 N.D. -0.5 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.1 -0.2 N.D. -0.3 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.5 +0.3 N.D. +0.1 N.D.
growth on ypd YPD +0.3 -0.5 N.D. +0.1 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.3 -0.4 N.D. +0.0 N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.1 +0.0 N.D. -0.1 N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.3 -0.3 N.D. -0.0 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.1 -0.0 N.D. +0.1 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.2 +0.1 N.D. -0.2 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 +0.4 N.D. -0.4 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 +0.4 N.D. -0.2 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.0 +0.2 N.D. +0.2 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.0 +0.2 N.D. +0.1 N.D.
growth on ricinoleic acid YNB Glucose +0.2 +0.6 N.D. -0.0 N.D.
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