Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10328 and 10329 are separated by 432 nucleotides10329 and 10330 are separated by 339 nucleotides10330 and 10331 are separated by 602 nucleotides10331 and 10332 are separated by 495 nucleotides mRNA_1960: 10328 - KOG0255 Synaptic vesicle transporter SVOP and related transporters (major facilitator superfamily), at 613,593 to 615,935 10328 mRNA_1961: 10329 - HMMPfam-CUE domain-PF02845,ProSiteProfiles-CUE domain profile.-PS51140, at 616,368 to 617,254 10329 mRNA_1962: 10330 - K00133 asd aspartate-semialdehyde dehydrogenase, at 617,594 to 618,818 10330 mRNA_1963: 10331 - K00767 nadC, QPRT nicotinate-nucleotide pyrophosphorylase (carboxylating), at 619,421 to 620,670 10331 mRNA_1964: 10332 - K01495 GCH1, folE GTP cyclohydrolase I, at 621,166 to 622,157 10332
Group Condition mRNA_1960 mRNA_1961 mRNA_1962 mRNA_1963 mRNA_1964
10328 10329 10330 10331 10332
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 -2.2 N.D. -1.4 N.D.
growth on oleic acid YNB Oleic Acid -0.4 -2.4 N.D. -0.3 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 -2.7 N.D. -0.5 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -0.3 -2.0 N.D. -0.2 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 +0.0 N.D. -1.9 N.D.
growth on oleic acid YNB Glucose -0.3 -0.7 N.D. -0.3 N.D.
growth on ypd YPD -0.4 -0.4 N.D. -0.4 N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.2 -0.9 N.D. -0.2 N.D.
auxotrophy Dropout Complete -0.1 -0.1 N.D. -0.4 N.D.
growth on ricinoleic acid YNB Glucose -0.3 -0.3 N.D. +0.2 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 +0.2 N.D. -0.5 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.1 -0.2 N.D. -0.2 N.D.
ypd buoyancy 40 hrs High Buoyancy -0.2 +0.2 N.D. -0.2 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 +0.3 N.D. -0.6 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.1 +0.1 N.D. -0.3 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.5 +0.3 N.D. +0.3 N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.1 +0.1 N.D. +0.2 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.1 +0.3 N.D. +0.1 N.D.
auxotrophy YNB Glucose -0.0 +0.3 N.D. +0.1 N.D.
facs on lipid accumulation Sorted High BODIPY Gate +0.2 +0.7 N.D. -0.2 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.0 +0.6 N.D. +0.5 N.D.
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