Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10329 and 10330 are separated by 339 nucleotides10330 and 10331 are separated by 602 nucleotides10331 and 10332 are separated by 495 nucleotides10332 and 10333 are separated by 563 nucleotides mRNA_1961: 10329 - HMMPfam-CUE domain-PF02845,ProSiteProfiles-CUE domain profile.-PS51140, at 616,368 to 617,254 10329 mRNA_1962: 10330 - K00133 asd aspartate-semialdehyde dehydrogenase, at 617,594 to 618,818 10330 mRNA_1963: 10331 - K00767 nadC, QPRT nicotinate-nucleotide pyrophosphorylase (carboxylating), at 619,421 to 620,670 10331 mRNA_1964: 10332 - K01495 GCH1, folE GTP cyclohydrolase I, at 621,166 to 622,157 10332 mRNA_1965: 10333 - K11422 SETD1, SET1 histone-lysine N-methyltransferase SETD1, at 622,721 to 626,978 10333
Group Condition mRNA_1961 mRNA_1962 mRNA_1963 mRNA_1964 mRNA_1965
10329 10330 10331 10332 10333
facs on lipid accumulation Sorted Low BODIPY Gate -2.7 N.D. -0.5 N.D. -3.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 N.D. -1.9 N.D. -1.6
facs on lipid accumulation Sorted No BODIPY Gate -0.9 N.D. -0.2 N.D. -1.7
growth on oleic acid YNB Oleic Acid -2.4 N.D. -0.3 N.D. +0.3
growth on ricinoleic acid YNB Ricinoleic Acid -2.2 N.D. -1.4 N.D. +1.4
growth on ypd YPD -0.4 N.D. -0.4 N.D. -1.0
facs on lipid accumulation Sorted High BODIPY Gate +0.7 N.D. -0.2 N.D. -2.0
growth on ricinoleic acid YNB Methylricinoleic Acid -2.0 N.D. -0.2 N.D. +1.1
growth on oleic acid YNB Glucose -0.7 N.D. -0.3 N.D. +0.4
auxotrophy Dropout Complete -0.1 N.D. -0.4 N.D. +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 N.D. -0.6 N.D. -0.1
ypd buoyancy 40 hrs Low Buoyancy +0.3 N.D. +0.1 N.D. -0.6
auxotrophy YNB Glucose 75mM Arginine +0.1 N.D. -0.3 N.D. +0.1
growth on ricinoleic acid YNB Glucose -0.3 N.D. +0.2 N.D. +0.2
auxotrophy YNB Glucose 75mM Methionine -0.2 N.D. -0.2 N.D. +0.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 N.D. -0.5 N.D. +0.6
ypd buoyancy 40 hrs High Buoyancy +0.2 N.D. -0.2 N.D. +0.3
ypd buoyancy 40 hrs Median Buoyancy +0.1 N.D. +0.2 N.D. +0.3
auxotrophy YNB Glucose +0.3 N.D. +0.1 N.D. +0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.6 N.D. +0.5 N.D. +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.3 N.D. +0.3 N.D. +1.4
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