Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10330 and 10331 are separated by 602 nucleotides10331 and 10332 are separated by 495 nucleotides10332 and 10333 are separated by 563 nucleotides10333 and 10334 are separated by 1207 nucleotides mRNA_1962: 10330 - K00133 asd aspartate-semialdehyde dehydrogenase, at 617,594 to 618,818 10330 mRNA_1963: 10331 - K00767 nadC, QPRT nicotinate-nucleotide pyrophosphorylase (carboxylating), at 619,421 to 620,670 10331 mRNA_1964: 10332 - K01495 GCH1, folE GTP cyclohydrolase I, at 621,166 to 622,157 10332 mRNA_1965: 10333 - K11422 SETD1, SET1 histone-lysine N-methyltransferase SETD1, at 622,721 to 626,978 10333 mRNA_1966: 10334 - K09522 DNAJC2 DnaJ homolog subfamily C member 2, at 628,186 to 629,960 10334
Group Condition mRNA_1962 mRNA_1963 mRNA_1964 mRNA_1965 mRNA_1966
10330 10331 10332 10333 10334
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.5 N.D. -3.1 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -1.9 N.D. -1.6 N.D.
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.2 N.D. -2.0 N.D.
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.2 N.D. -1.7 N.D.
growth on ypd YPD N.D. -0.4 N.D. -1.0 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.6 N.D. -0.1 N.D.
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.1 N.D. -0.6 N.D.
auxotrophy Dropout Complete N.D. -0.4 N.D. +0.0 N.D.
auxotrophy YNB Glucose 75mM Arginine N.D. -0.3 N.D. +0.1 N.D.
growth on oleic acid YNB Oleic Acid N.D. -0.3 N.D. +0.3 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -1.4 N.D. +1.4 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.5 N.D. +0.6 N.D.
ypd buoyancy 40 hrs High Buoyancy N.D. -0.2 N.D. +0.3 N.D.
growth on oleic acid YNB Glucose N.D. -0.3 N.D. +0.4 N.D.
auxotrophy YNB Glucose N.D. +0.1 N.D. +0.2 N.D.
auxotrophy YNB Glucose 75mM Methionine N.D. -0.2 N.D. +0.5 N.D.
growth on ricinoleic acid YNB Glucose N.D. +0.2 N.D. +0.2 N.D.
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.2 N.D. +0.3 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.5 N.D. +0.3 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.2 N.D. +1.1 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.3 N.D. +1.4 N.D.
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