Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10332 and 10333 are separated by 563 nucleotides10333 and 10334 are separated by 1207 nucleotides10334 and 10335 are separated by 303 nucleotides10335 and 10336 are separated by 391 nucleotides mRNA_1964: 10332 - K01495 GCH1, folE GTP cyclohydrolase I, at 621,166 to 622,157 10332 mRNA_1965: 10333 - K11422 SETD1, SET1 histone-lysine N-methyltransferase SETD1, at 622,721 to 626,978 10333 mRNA_1966: 10334 - K09522 DNAJC2 DnaJ homolog subfamily C member 2, at 628,186 to 629,960 10334 mRNA_1967: 10335 - BLAST proteophosphoglycan 5 [Moniliophthora roreri MCA ..., at 630,264 to 633,467 10335 mRNA_1968: 10336 - HMMPfam-Membrane bound O-acyl transferase family-PF13813, at 633,859 to 635,339 10336
Group Condition mRNA_1964 mRNA_1965 mRNA_1966 mRNA_1967 mRNA_1968
10332 10333 10334 10335 10336
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -3.1 N.D. -0.4 -0.6
facs on lipid accumulation Sorted High BODIPY Gate N.D. -2.0 N.D. -0.1 +0.4
facs on lipid accumulation Sorted No BODIPY Gate N.D. -1.7 N.D. -0.2 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -1.6 N.D. -0.0 +0.5
growth on ypd YPD N.D. -1.0 N.D. +0.2 +0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.6 N.D. +0.1 +0.2
growth on oleic acid YNB Glucose N.D. +0.4 N.D. -0.2 -0.4
growth on ricinoleic acid YNB Glucose N.D. +0.2 N.D. -0.1 -0.3
auxotrophy Dropout Complete N.D. +0.0 N.D. -0.2 +0.0
growth on oleic acid YNB Oleic Acid N.D. +0.3 N.D. -0.1 -0.1
auxotrophy YNB Glucose 75mM Arginine N.D. +0.1 N.D. -0.1 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.6 N.D. -0.1 -0.3
ypd buoyancy 40 hrs High Buoyancy N.D. +0.3 N.D. +0.2 -0.2
auxotrophy YNB Glucose N.D. +0.2 N.D. -0.1 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.1 N.D. +0.2 +0.3
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.3 N.D. +0.2 -0.0
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.3 N.D. +0.1 +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +1.4 N.D. +0.0 -0.6
auxotrophy YNB Glucose 75mM Methionine N.D. +0.5 N.D. +0.1 +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +1.1 N.D. +0.1 +0.2
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +1.4 N.D. +0.2 +0.4
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