Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10338 and 10339 are separated by 2423 nucleotides10339 and 10340 are separated by 539 nucleotides10340 and 10341 are separated by 370 nucleotides10341 and 10342 are separated by 226 nucleotides mRNA_1970: 10338 - KOG2614 Kynurenine 3-monooxygenase and related flavoprotein monooxygenases, at 638,087 to 640,268 10338 mRNA_1971: 10339 - K02429 fucP MFS transporter, FHS family, L-fucose permease, at 642,692 to 644,646 10339 mRNA_1972: 10340 - K20301 TRAPPC2, TRS20 trafficking protein particle complex subunit 2, at 645,186 to 645,812 10340 mRNA_1973: 10341 - KOG1057 Arp2/3 complex-interacting protein VIP1/Asp1, involved in regulation of actin cytoskeleton, at 646,183 to 650,027 10341 mRNA_1974: 10342 - KOG4067 Uncharacterized conserved protein, at 650,254 to 651,223 10342
Group Condition mRNA_1970 mRNA_1971 mRNA_1972 mRNA_1973 mRNA_1974
10338 10339 10340 10341 10342
growth on ricinoleic acid YNB Glucose +0.2 -0.2 N.D. -0.6 -0.7
growth on oleic acid YNB Glucose -0.2 -0.3 N.D. -0.6 -0.3
ypd buoyancy 40 hrs No Buoyancy Separation -0.8 -0.5 N.D. -0.1 +0.2
growth on ricinoleic acid YNB Ricinoleic Acid +0.5 -0.4 N.D. -1.1 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid +0.4 -0.5 N.D. -0.2 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 -0.4 N.D. +0.3 -0.2
growth on oleic acid YNB Oleic Acid -0.1 -0.1 N.D. -0.0 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 +0.2 N.D. +0.2 -0.7
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 +0.5 N.D. -0.1 -0.6
auxotrophy YNB Glucose +0.0 -0.6 N.D. +0.2 +0.5
facs on lipid accumulation Sorted Low BODIPY Gate +0.0 -0.5 N.D. +0.4 +0.3
growth on ypd YPD -0.1 +0.3 N.D. -0.3 +0.4
facs on lipid accumulation Sorted High BODIPY Gate -0.0 +0.4 N.D. +0.7 -0.8
auxotrophy Dropout Complete +0.2 -0.3 N.D. +0.2 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 +0.3 N.D. +0.2 -0.3
ypd buoyancy 40 hrs Median Buoyancy +0.0 +0.1 N.D. +0.2 +0.2
ypd buoyancy 40 hrs High Buoyancy +0.1 +0.2 N.D. +0.2 +0.2
ypd buoyancy 40 hrs Low Buoyancy +0.2 +0.4 N.D. +0.1 -0.1
auxotrophy YNB Glucose 75mM Arginine +0.2 +0.1 N.D. +0.3 +0.4
auxotrophy YNB Glucose 75mM Methionine +0.5 -0.0 N.D. +0.2 +0.4
facs on lipid accumulation Sorted No BODIPY Gate +0.4 +0.5 N.D. +0.1 +0.2
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