Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10344 and 10345 are separated by 173 nucleotides10345 and 10346 are separated by 211 nucleotides10346 and 10347 are separated by 406 nucleotides10347 and 10348 are separated by 400 nucleotides mRNA_1976: 10344 - Hypothetical Protein, at 655,294 to 656,131 10344 mRNA_1977: 10345 - HMMPfam-Eukaryotic initiation factor 4E-PF01652,SUPERFAMILY--SSF55418, at 656,305 to 657,635 10345 mRNA_1978: 10346 - K14409 SMG7, EST1C protein SMG7, at 657,847 to 661,571 10346 mRNA_1979: 10347 - KOG2362 Uncharacterized Fe-S protein, at 661,978 to 663,393 10347 mRNA_1980: 10348 - KOG3436 60S ribosomal protein L35, at 663,794 to 664,282 10348
Group Condition mRNA_1976 mRNA_1977 mRNA_1978 mRNA_1979 mRNA_1980
10344 10345 10346 10347 10348
facs on lipid accumulation Sorted Low BODIPY Gate -2.6 N.D. +1.0 +0.3 N.D.
growth on ypd YPD -0.8 N.D. +0.0 -0.3 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.9 N.D. -0.2 +0.3 N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.3 N.D. -0.1 +0.1 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -1.1 N.D. +0.6 +0.2 N.D.
growth on oleic acid YNB Glucose -0.4 N.D. +0.3 -0.2 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.9 N.D. -1.0 -0.1 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.1 N.D. +0.0 -0.2 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.4 N.D. +0.5 +0.1 N.D.
facs on lipid accumulation Sorted High BODIPY Gate +0.0 N.D. +0.0 +0.1 N.D.
auxotrophy YNB Glucose 75mM Methionine -0.0 N.D. +0.6 -0.1 N.D.
auxotrophy YNB Glucose 75mM Arginine -0.1 N.D. +0.7 -0.1 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 N.D. +0.6 -0.2 N.D.
growth on oleic acid YNB Oleic Acid -0.0 N.D. +0.7 -0.0 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 N.D. +0.5 -0.1 N.D.
growth on ricinoleic acid YNB Glucose +0.7 N.D. +0.2 -0.1 N.D.
auxotrophy Dropout Complete +0.1 N.D. +1.0 -0.2 N.D.
auxotrophy YNB Glucose +0.4 N.D. +0.6 -0.1 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.7 N.D. +0.3 -0.0 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.9 N.D. +0.4 -0.1 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +1.1 N.D. +0.8 -0.1 N.D.
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