Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10347 and 10348 are separated by 400 nucleotides10348 and 10349 are separated by 791 nucleotides10349 and 10350 are separated by 367 nucleotides10350 and 10351 are separated by 935 nucleotides mRNA_1979: 10347 - KOG2362 Uncharacterized Fe-S protein, at 661,978 to 663,393 10347 mRNA_1980: 10348 - KOG3436 60S ribosomal protein L35, at 663,794 to 664,282 10348 mRNA_1981: 10349 - HMMPfam-Glycosyl hydrolases family 16-PF00722,SUPERFAMILY--SSF49899, at 665,074 to 666,880 10349 mRNA_1982: 10350 - HMMPfam-Copper binding proteins, plastocyanin/azurin family-PF00127,ProSiteProfiles-Prokaryotic membrane lipoprotein lipid attachment site profile.-PS51257,SUPERFAMILY--SSF49503, at 667,248 to 669,066 10350 mRNA_1983: 10351 - K07213 ATOX1, ATX1, copZ, golB copper chaperone, at 670,002 to 670,488 10351
Group Condition mRNA_1979 mRNA_1980 mRNA_1981 mRNA_1982 mRNA_1983
10347 10348 10349 10350 10351
facs on lipid accumulation Sorted High BODIPY Gate +0.1 N.D. -0.4 -0.3 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.0 N.D. -0.4 -0.1 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 N.D. -0.1 +0.0 N.D.
ypd buoyancy 40 hrs High Buoyancy -0.2 N.D. +0.1 -0.2 N.D.
growth on ricinoleic acid YNB Glucose -0.1 N.D. -0.1 +0.0 N.D.
growth on ypd YPD -0.3 N.D. +0.1 -0.1 N.D.
auxotrophy Dropout Complete -0.2 N.D. -0.9 +1.0 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 N.D. +0.1 -0.1 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 N.D. -0.2 +0.3 N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.2 N.D. -0.1 -0.0 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.1 N.D. +0.2 -0.2 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 N.D. -0.1 +0.4 N.D.
auxotrophy YNB Glucose 75mM Arginine -0.1 N.D. -0.6 +0.9 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 N.D. +0.1 +0.3 N.D.
growth on oleic acid YNB Glucose -0.2 N.D. +0.2 +0.3 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.3 N.D. +0.1 +0.2 N.D.
auxotrophy YNB Glucose 75mM Methionine -0.1 N.D. -0.3 +1.0 N.D.
growth on oleic acid YNB Oleic Acid -0.0 N.D. +0.3 +0.4 N.D.
auxotrophy YNB Glucose -0.1 N.D. -0.4 +1.2 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.1 N.D. +0.8 +0.1 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 N.D. +0.3 +0.4 N.D.
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