Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10348 and 10349 are separated by 791 nucleotides10349 and 10350 are separated by 367 nucleotides10350 and 10351 are separated by 935 nucleotides10351 and 10352 are separated by 141 nucleotides mRNA_1980: 10348 - KOG3436 60S ribosomal protein L35, at 663,794 to 664,282 10348 mRNA_1981: 10349 - HMMPfam-Glycosyl hydrolases family 16-PF00722,SUPERFAMILY--SSF49899, at 665,074 to 666,880 10349 mRNA_1982: 10350 - HMMPfam-Copper binding proteins, plastocyanin/azurin family-PF00127,ProSiteProfiles-Prokaryotic membrane lipoprotein lipid attachment site profile.-PS51257,SUPERFAMILY--SSF49503, at 667,248 to 669,066 10350 mRNA_1983: 10351 - K07213 ATOX1, ATX1, copZ, golB copper chaperone, at 670,002 to 670,488 10351 mRNA_1984: 10352 - Hypothetical Protein, at 670,630 to 671,905 10352
Group Condition mRNA_1980 mRNA_1981 mRNA_1982 mRNA_1983 mRNA_1984
10348 10349 10350 10351 10352
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.1 -0.1 N.D. -0.6
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.4 -0.1 N.D. -0.0
growth on ypd YPD N.D. +0.1 -0.1 N.D. -0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.2 +0.3 N.D. -0.4
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.4 -0.3 N.D. +0.4
auxotrophy Dropout Complete N.D. -0.9 +1.0 N.D. -0.3
ypd buoyancy 40 hrs High Buoyancy N.D. +0.1 -0.2 N.D. -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.1 +0.0 N.D. +0.0
growth on ricinoleic acid YNB Glucose N.D. -0.1 +0.0 N.D. +0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.1 -0.0 N.D. +0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.2 -0.2 N.D. -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.1 +0.4 N.D. -0.1
auxotrophy YNB Glucose 75mM Methionine N.D. -0.3 +1.0 N.D. -0.5
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.1 +0.3 N.D. -0.1
auxotrophy YNB Glucose 75mM Arginine N.D. -0.6 +0.9 N.D. +0.0
growth on oleic acid YNB Oleic Acid N.D. +0.3 +0.4 N.D. -0.4
growth on oleic acid YNB Glucose N.D. +0.2 +0.3 N.D. +0.0
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.1 +0.2 N.D. +0.4
auxotrophy YNB Glucose N.D. -0.4 +1.2 N.D. -0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.8 +0.1 N.D. -0.0
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.3 +0.4 N.D. +0.7
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