Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10350 and 10351 are separated by 935 nucleotides10351 and 10352 are separated by 141 nucleotides10352 and 10353 are separated by 672 nucleotides10353 and 10354 are separated by 1107 nucleotides mRNA_1982: 10350 - HMMPfam-Copper binding proteins, plastocyanin/azurin family-PF00127,ProSiteProfiles-Prokaryotic membrane lipoprotein lipid attachment site profile.-PS51257,SUPERFAMILY--SSF49503, at 667,248 to 669,066 10350 mRNA_1983: 10351 - K07213 ATOX1, ATX1, copZ, golB copper chaperone, at 670,002 to 670,488 10351 mRNA_1984: 10352 - Hypothetical Protein, at 670,630 to 671,905 10352 mRNA_1985: 10353 - Hypothetical Protein, at 672,578 to 672,870 10353 mRNA_1986: 10354 - KOG2458 Endoplasmic reticulum protein EP58, contains filamin rod domain and KDEL motif, at 673,978 to 676,416 10354
Group Condition mRNA_1982 mRNA_1983 mRNA_1984 mRNA_1985 mRNA_1986
10350 10351 10352 10353 10354
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 N.D. -0.6 N.D. N.D.
growth on ypd YPD -0.1 N.D. -0.4 N.D. N.D.
ypd buoyancy 40 hrs High Buoyancy -0.2 N.D. -0.1 N.D. N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.2 N.D. -0.1 N.D. N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 N.D. -0.0 N.D. N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.3 N.D. -0.4 N.D. N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.0 N.D. +0.0 N.D. N.D.
growth on oleic acid YNB Oleic Acid +0.4 N.D. -0.4 N.D. N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.1 N.D. -0.0 N.D. N.D.
growth on ricinoleic acid YNB Glucose +0.0 N.D. +0.1 N.D. N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.0 N.D. +0.1 N.D. N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 N.D. -0.1 N.D. N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.3 N.D. +0.4 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.4 N.D. -0.1 N.D. N.D.
growth on oleic acid YNB Glucose +0.3 N.D. +0.0 N.D. N.D.
auxotrophy YNB Glucose 75mM Methionine +1.0 N.D. -0.5 N.D. N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 N.D. +0.4 N.D. N.D.
auxotrophy Dropout Complete +1.0 N.D. -0.3 N.D. N.D.
auxotrophy YNB Glucose 75mM Arginine +0.9 N.D. +0.0 N.D. N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.4 N.D. +0.7 N.D. N.D.
auxotrophy YNB Glucose +1.2 N.D. -0.1 N.D. N.D.
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