Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10351 and 10352 are separated by 141 nucleotides10352 and 10353 are separated by 672 nucleotides10353 and 10354 are separated by 1107 nucleotides10354 and 10355 are separated by 690 nucleotides mRNA_1983: 10351 - K07213 ATOX1, ATX1, copZ, golB copper chaperone, at 670,002 to 670,488 10351 mRNA_1984: 10352 - Hypothetical Protein, at 670,630 to 671,905 10352 mRNA_1985: 10353 - Hypothetical Protein, at 672,578 to 672,870 10353 mRNA_1986: 10354 - KOG2458 Endoplasmic reticulum protein EP58, contains filamin rod domain and KDEL motif, at 673,978 to 676,416 10354 mRNA_1987: 10355 - KOG0255 Synaptic vesicle transporter SVOP and related transporters (major facilitator superfamily), at 677,107 to 679,198 10355
Group Condition mRNA_1983 mRNA_1984 mRNA_1985 mRNA_1986 mRNA_1987
10351 10352 10353 10354 10355
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.6 N.D. N.D. -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.4 N.D. N.D. -0.3
auxotrophy YNB Glucose 75mM Methionine N.D. -0.5 N.D. N.D. +0.1
growth on oleic acid YNB Oleic Acid N.D. -0.4 N.D. N.D. +0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.0 N.D. N.D. -0.2
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.1 N.D. N.D. -0.0
growth on ypd YPD N.D. -0.4 N.D. N.D. +0.4
auxotrophy Dropout Complete N.D. -0.3 N.D. N.D. +0.3
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.7 N.D. N.D. -0.6
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.1 N.D. N.D. +0.2
ypd buoyancy 40 hrs High Buoyancy N.D. -0.1 N.D. N.D. +0.2
auxotrophy YNB Glucose 75mM Arginine N.D. +0.0 N.D. N.D. +0.1
auxotrophy YNB Glucose N.D. -0.1 N.D. N.D. +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.0 N.D. N.D. +0.2
growth on oleic acid YNB Glucose N.D. +0.0 N.D. N.D. +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.1 N.D. N.D. +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.0 N.D. N.D. +0.4
growth on ricinoleic acid YNB Glucose N.D. +0.1 N.D. N.D. +0.3
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.1 N.D. N.D. +0.6
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.4 N.D. N.D. +0.4
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.4 N.D. N.D. +0.7
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