Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10354 and 10355 are separated by 690 nucleotides10355 and 10356 are separated by 59 nucleotides10356 and 10357 are separated by 1630 nucleotides10357 and 10358 are separated by 116 nucleotides mRNA_1986: 10354 - KOG2458 Endoplasmic reticulum protein EP58, contains filamin rod domain and KDEL motif, at 673,978 to 676,416 10354 mRNA_1987: 10355 - KOG0255 Synaptic vesicle transporter SVOP and related transporters (major facilitator superfamily), at 677,107 to 679,198 10355 mRNA_1988: 10356 - KOG1674 Cyclin, at 679,258 to 681,059 10356 mRNA_1989: 10357 - KOG0551 Hsp90 co-chaperone CNS1 (contains TPR repeats), at 682,690 to 684,207 10357 mRNA_1990: 10358 - HMMPfam-PPR repeat-PF01535,HMMPfam-Pentatricopeptide repeat domain-PF13812,ProSiteProfiles-Pentatricopeptide (PPR) repeat profile.-PS51375,TIGRFAM-PPR pentatricopeptide repeat domain-TIGR00756, at 684,324 to 686,633 10358
Group Condition mRNA_1986 mRNA_1987 mRNA_1988 mRNA_1989 mRNA_1990
10354 10355 10356 10357 10358
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.6 -0.3 N.D. N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.3 -0.6 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.2 -0.2 N.D. N.D.
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.0 -0.4 N.D. N.D.
growth on ypd YPD N.D. +0.4 -0.8 N.D. N.D.
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.2 -0.0 N.D. N.D.
ypd buoyancy 40 hrs High Buoyancy N.D. +0.2 -0.4 N.D. N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.2 -0.2 N.D. N.D.
auxotrophy YNB Glucose 75mM Arginine N.D. +0.1 -0.1 N.D. N.D.
growth on oleic acid YNB Oleic Acid N.D. +0.1 -0.0 N.D. N.D.
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.4 -0.4 N.D. N.D.
growth on ricinoleic acid YNB Glucose N.D. +0.3 -0.2 N.D. N.D.
auxotrophy YNB Glucose 75mM Methionine N.D. +0.1 -0.0 N.D. N.D.
auxotrophy YNB Glucose N.D. +0.3 -0.1 N.D. N.D.
auxotrophy Dropout Complete N.D. +0.3 -0.1 N.D. N.D.
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.2 -0.0 N.D. N.D.
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.6 -0.3 N.D. N.D.
growth on oleic acid YNB Glucose N.D. +0.2 +0.1 N.D. N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.4 +0.0 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.3 +0.1 N.D. N.D.
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.7 +0.0 N.D. N.D.
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