Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10356 and 10357 are separated by 1630 nucleotides10357 and 10358 are separated by 116 nucleotides10358 and 10359 are separated by 924 nucleotides10359 and 10360 are separated by 385 nucleotides mRNA_1988: 10356 - KOG1674 Cyclin, at 679,258 to 681,059 10356 mRNA_1989: 10357 - KOG0551 Hsp90 co-chaperone CNS1 (contains TPR repeats), at 682,690 to 684,207 10357 mRNA_1990: 10358 - HMMPfam-PPR repeat-PF01535,HMMPfam-Pentatricopeptide repeat domain-PF13812,ProSiteProfiles-Pentatricopeptide (PPR) repeat profile.-PS51375,TIGRFAM-PPR pentatricopeptide repeat domain-TIGR00756, at 684,324 to 686,633 10358 mRNA_1991: 10359 - K03457 TC.NCS1 nucleobase-cation symporter-1, NCS1 family, at 687,558 to 690,393 10359 mRNA_1992: 10360 - BLAST protein of unknown function [Taphrina deformans PYCC ..., at 690,779 to 693,096 10360
Group Condition mRNA_1988 mRNA_1989 mRNA_1990 mRNA_1991 mRNA_1992
10356 10357 10358 10359 10360
facs on lipid accumulation Sorted High BODIPY Gate -0.4 N.D. N.D. -0.6 -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.6 N.D. N.D. -0.0 -0.4
growth on ypd YPD -0.8 N.D. N.D. +0.1 -0.1
ypd buoyancy 40 hrs High Buoyancy -0.4 N.D. N.D. -0.2 -0.1
ypd buoyancy 40 hrs Low Buoyancy -0.0 N.D. N.D. -0.2 -0.3
ypd buoyancy 40 hrs Median Buoyancy -0.4 N.D. N.D. -0.1 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation -0.3 N.D. N.D. +0.1 -0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.3 N.D. N.D. -0.2 +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 N.D. N.D. +0.2 -0.2
growth on ricinoleic acid YNB Glucose -0.2 N.D. N.D. +0.1 +0.0
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 N.D. N.D. +0.3 -0.2
auxotrophy Dropout Complete -0.1 N.D. N.D. -0.1 +0.2
facs on lipid accumulation Sorted Low BODIPY Gate +0.0 N.D. N.D. -0.4 +0.5
auxotrophy YNB Glucose 75mM Arginine -0.1 N.D. N.D. -0.1 +0.3
auxotrophy YNB Glucose -0.1 N.D. N.D. -0.1 +0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.0 N.D. N.D. +0.1 +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 N.D. N.D. +0.2 -0.1
growth on oleic acid YNB Oleic Acid -0.0 N.D. N.D. +0.5 +0.0
growth on oleic acid YNB Glucose +0.1 N.D. N.D. +0.3 +0.0
growth on ricinoleic acid YNB Ricinoleic Acid -0.0 N.D. N.D. +0.6 -0.0
auxotrophy YNB Glucose 75mM Methionine -0.0 N.D. N.D. +0.0 +0.6
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