Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10362 and 10363 are separated by 930 nucleotides10363 and 10364 are separated by 219 nucleotides10364 and 10365 are separated by 600 nucleotides10365 and 10366 are separated by 487 nucleotides mRNA_1994: 10362 - K03235 EF3, TEF3 elongation factor 3, at 695,063 to 698,759 10362 mRNA_1995: 10363 - KOG0062 ATPase component of ABC transporters with duplicated ATPase domains/Translation elongation factor EF-3b, at 699,690 to 700,377 10363 mRNA_1996: 10364 - KOG0379 Kelch repeat-containing proteins, at 700,597 to 701,909 10364 mRNA_1997: 10365 - KOG0627 Heat shock transcription factor, at 702,510 to 703,691 10365 mRNA_1998: 10366 - K08869 ADCK, ABC1 aarF domain-containing kinase, at 704,179 to 706,336 10366
Group Condition mRNA_1994 mRNA_1995 mRNA_1996 mRNA_1997 mRNA_1998
10362 10363 10364 10365 10366
growth on oleic acid YNB Oleic Acid N.D. -0.5 -0.5 N.D. -0.2
growth on oleic acid YNB Glucose N.D. -0.6 -0.6 N.D. -0.0
auxotrophy YNB Glucose 75mM Arginine N.D. -0.3 -0.5 N.D. -0.3
auxotrophy Dropout Complete N.D. -0.1 -0.5 N.D. -0.4
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.1 -0.3 N.D. -0.7
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.3 -0.0 N.D. -0.4
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.1 +0.0 N.D. -0.8
auxotrophy YNB Glucose N.D. +0.0 -0.8 N.D. +0.1
growth on ypd YPD N.D. +0.1 -0.7 N.D. +0.1
growth on ricinoleic acid YNB Glucose N.D. -0.3 -0.3 N.D. +0.2
ypd buoyancy 40 hrs High Buoyancy N.D. +0.2 -0.3 N.D. -0.3
auxotrophy YNB Glucose 75mM Methionine N.D. +0.2 -0.6 N.D. +0.2
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.5 +0.3 N.D. -0.0
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.0 -0.2 N.D. +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.1 -0.1 N.D. +0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.5 +0.0 N.D. -0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.1 +0.7 N.D. +0.0
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.8 +0.1 N.D. -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.6 +0.1 N.D. -0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.4 +0.2 N.D. +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.7 +0.1 N.D. +0.0
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