Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10363 and 10364 are separated by 219 nucleotides10364 and 10365 are separated by 600 nucleotides10365 and 10366 are separated by 487 nucleotides10366 and 10367 are separated by 451 nucleotides mRNA_1995: 10363 - KOG0062 ATPase component of ABC transporters with duplicated ATPase domains/Translation elongation factor EF-3b, at 699,690 to 700,377 10363 mRNA_1996: 10364 - KOG0379 Kelch repeat-containing proteins, at 700,597 to 701,909 10364 mRNA_1997: 10365 - KOG0627 Heat shock transcription factor, at 702,510 to 703,691 10365 mRNA_1998: 10366 - K08869 ADCK, ABC1 aarF domain-containing kinase, at 704,179 to 706,336 10366 mRNA_1999: 10367 - Hypothetical Protein, at 706,788 to 707,404 10367
Group Condition mRNA_1995 mRNA_1996 mRNA_1997 mRNA_1998 mRNA_1999
10363 10364 10365 10366 10367
auxotrophy Dropout Complete -0.1 -0.5 N.D. -0.4 -2.0
auxotrophy YNB Glucose 75mM Methionine +0.2 -0.6 N.D. +0.2 -2.0
growth on oleic acid YNB Oleic Acid -0.5 -0.5 N.D. -0.2 -0.8
growth on oleic acid YNB Glucose -0.6 -0.6 N.D. -0.0 -0.7
auxotrophy YNB Glucose +0.0 -0.8 N.D. +0.1 -1.1
auxotrophy YNB Glucose 75mM Arginine -0.3 -0.5 N.D. -0.3 -0.5
growth on ricinoleic acid YNB Glucose -0.3 -0.3 N.D. +0.2 -1.0
growth on ricinoleic acid YNB Methylricinoleic Acid -0.3 -0.0 N.D. -0.4 -0.5
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 -0.1 N.D. +0.1 -1.3
facs on lipid accumulation Sorted No BODIPY Gate +0.5 +0.0 N.D. -0.2 -1.0
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 -0.3 N.D. -0.7 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.7 +0.1 N.D. +0.0 -1.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.6 +0.1 N.D. -0.0 -1.2
ypd buoyancy 40 hrs High Buoyancy +0.2 -0.3 N.D. -0.3 -0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.8 +0.1 N.D. -0.3 -1.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.4 +0.2 N.D. +0.2 -1.0
ypd buoyancy 40 hrs Median Buoyancy -0.5 +0.3 N.D. -0.0 +0.3
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 +0.0 N.D. -0.8 +0.9
growth on ricinoleic acid YNB Ricinoleic Acid -0.0 -0.2 N.D. +0.2 +0.3
growth on ypd YPD +0.1 -0.7 N.D. +0.1 +0.7
ypd buoyancy 40 hrs Low Buoyancy -0.1 +0.7 N.D. +0.0 +0.3
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