Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10365 and 10366 are separated by 487 nucleotides10366 and 10367 are separated by 451 nucleotides10367 and 10368 are separated by 236 nucleotides10368 and 10369 are separated by 113 nucleotides mRNA_1997: 10365 - KOG0627 Heat shock transcription factor, at 702,510 to 703,691 10365 mRNA_1998: 10366 - K08869 ADCK, ABC1 aarF domain-containing kinase, at 704,179 to 706,336 10366 mRNA_1999: 10367 - Hypothetical Protein, at 706,788 to 707,404 10367 mRNA_2000: 10368 - K09650 PARL, PSARL, PCP1 rhomboid-like protein, at 707,641 to 708,923 10368 mRNA_2001: 10369 - Hypothetical Protein, at 709,037 to 710,403 10369
Group Condition mRNA_1997 mRNA_1998 mRNA_1999 mRNA_2000 mRNA_2001
10365 10366 10367 10368 10369
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.0 -1.4 -0.3 -0.7
auxotrophy Dropout Complete N.D. -0.4 -2.0 -0.1 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.1 -1.3 -0.3 -0.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.0 -1.2 -0.2 -0.4
auxotrophy YNB Glucose 75mM Methionine N.D. +0.2 -2.0 +0.0 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.8 +0.9 -0.1 -1.2
auxotrophy YNB Glucose N.D. +0.1 -1.1 +0.0 -0.2
growth on ricinoleic acid YNB Glucose N.D. +0.2 -1.0 +0.0 -0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.2 -1.0 -0.0 -0.2
ypd buoyancy 40 hrs High Buoyancy N.D. -0.3 -0.1 -0.2 -0.3
growth on oleic acid YNB Oleic Acid N.D. -0.2 -0.8 +0.3 -0.0
auxotrophy YNB Glucose 75mM Arginine N.D. -0.3 -0.5 +0.1 -0.1
growth on oleic acid YNB Glucose N.D. -0.0 -0.7 -0.1 +0.2
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.3 -1.1 +0.5 +0.2
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.2 -1.0 +0.4 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.4 -0.5 +0.2 +0.4
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.0 +0.3 -0.1 -0.3
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.7 +0.2 -0.1 +0.5
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.0 +0.3 +0.2 -0.2
growth on ypd YPD N.D. +0.1 +0.7 +0.0 +0.2
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.2 +0.3 +0.2 +0.8
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