Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10370 and 10371 are separated by 159 nucleotides10371 and 10372 are separated by 611 nucleotides10372 and 10373 are separated by 762 nucleotides10373 and 10374 are separated by 527 nucleotides mRNA_2002: 10370 - HMMPfam-NAD(P)-binding Rossmann-like domain-PF13450,SUPERFAMILY--SSF51971, at 712,088 to 714,509 10370 mRNA_2003: 10371 - KOG2458 Endoplasmic reticulum protein EP58, contains filamin rod domain and KDEL motif, at 714,669 to 717,655 10371 mRNA_2004: 10372 - Hypothetical Protein, at 718,267 to 719,987 10372 mRNA_2005: 10373 - K08286 E2.7.11.- protein-serine/threonine kinase, at 720,750 to 723,688 10373 mRNA_2006: 10374 - K00380 cysJ sulfite reductase (NADPH) flavoprotein alpha-component, at 724,216 to 728,973 10374
Group Condition mRNA_2002 mRNA_2003 mRNA_2004 mRNA_2005 mRNA_2006
10370 10371 10372 10373 10374
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -3.8 -0.1 +0.8 -1.6
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -2.0 -1.0 +0.2 -1.6
auxotrophy YNB Glucose 75mM Arginine N.D. +0.4 +0.5 -1.0 -2.2
auxotrophy YNB Glucose N.D. +0.7 +0.3 -1.1 -2.2
growth on ricinoleic acid YNB Glucose N.D. +1.0 -0.2 -0.8 -2.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.5 -0.5 -0.3 -0.6
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.1 -0.2 +0.3 -1.1
growth on oleic acid YNB Glucose N.D. +0.5 +0.3 -0.2 -1.6
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.3 -0.2 +0.6 -1.0
auxotrophy Dropout Complete N.D. +0.3 +0.2 -0.6 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +1.6 -0.0 -0.9 -1.0
ypd buoyancy 40 hrs High Buoyancy N.D. -0.8 +0.1 +0.6 -0.2
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +1.5 -0.3 -0.6 -0.8
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.5 -0.2 +0.2 +0.3
growth on ypd YPD N.D. -0.2 -0.0 +0.1 -0.1
growth on oleic acid YNB Oleic Acid N.D. +0.4 +0.2 +0.2 -0.9
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +1.0 -0.7 -1.0 +0.9
auxotrophy YNB Glucose 75mM Methionine N.D. +0.7 +0.4 -0.6 -0.2
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.2 +0.1 +0.6 -0.2
facs on lipid accumulation Sorted High BODIPY Gate N.D. +2.2 +0.3 -0.2 -0.4
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.6 +0.3 +1.0 +0.5
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