Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10371 and 10372 are separated by 611 nucleotides10372 and 10373 are separated by 762 nucleotides10373 and 10374 are separated by 527 nucleotides10374 and 10375 are separated by 238 nucleotides mRNA_2003: 10371 - KOG2458 Endoplasmic reticulum protein EP58, contains filamin rod domain and KDEL motif, at 714,669 to 717,655 10371 mRNA_2004: 10372 - Hypothetical Protein, at 718,267 to 719,987 10372 mRNA_2005: 10373 - K08286 E2.7.11.- protein-serine/threonine kinase, at 720,750 to 723,688 10373 mRNA_2006: 10374 - K00380 cysJ sulfite reductase (NADPH) flavoprotein alpha-component, at 724,216 to 728,973 10374 mRNA_2007: 10375 - K15331 TRMT2B, TRM2 tRNA (uracil-5-)-methyltransferase, at 729,212 to 731,398 10375
Group Condition mRNA_2003 mRNA_2004 mRNA_2005 mRNA_2006 mRNA_2007
10371 10372 10373 10374 10375
facs on lipid accumulation Sorted Low BODIPY Gate -3.8 -0.1 +0.8 -1.6 -0.8
buoyancy on lipid accumulation 40 hrs Low Buoyancy -2.0 -1.0 +0.2 -1.6 +0.2
auxotrophy YNB Glucose 75mM Arginine +0.4 +0.5 -1.0 -2.2 -0.3
growth on ricinoleic acid YNB Glucose +1.0 -0.2 -0.8 -2.0 -0.5
auxotrophy YNB Glucose +0.7 +0.3 -1.1 -2.2 -0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.3 -0.2 +0.6 -1.0 -0.9
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.5 -0.5 -0.3 -0.6 +0.2
growth on oleic acid YNB Glucose +0.5 +0.3 -0.2 -1.6 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 -0.2 +0.3 -1.1 +0.2
auxotrophy Dropout Complete +0.3 +0.2 -0.6 -0.2 -0.1
ypd buoyancy 40 hrs High Buoyancy -0.8 +0.1 +0.6 -0.2 -0.2
growth on ricinoleic acid YNB Ricinoleic Acid +1.5 -0.3 -0.6 -0.8 -0.1
growth on ypd YPD -0.2 -0.0 +0.1 -0.1 -0.2
growth on oleic acid YNB Oleic Acid +0.4 +0.2 +0.2 -0.9 -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid +1.6 -0.0 -0.9 -1.0 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation -0.5 -0.2 +0.2 +0.3 +0.2
ypd buoyancy 40 hrs Median Buoyancy -0.2 +0.1 +0.6 -0.2 -0.1
auxotrophy YNB Glucose 75mM Methionine +0.7 +0.4 -0.6 -0.2 +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy +1.0 -0.7 -1.0 +0.9 +0.2
facs on lipid accumulation Sorted High BODIPY Gate +2.2 +0.3 -0.2 -0.4 -0.8
ypd buoyancy 40 hrs Low Buoyancy +0.6 +0.3 +1.0 +0.5 -0.0
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