Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10373 and 10374 are separated by 527 nucleotides10374 and 10375 are separated by 238 nucleotides10375 and 10376 are separated by 253 nucleotides10376 and 10377 are separated by 847 nucleotides mRNA_2005: 10373 - K08286 E2.7.11.- protein-serine/threonine kinase, at 720,750 to 723,688 10373 mRNA_2006: 10374 - K00380 cysJ sulfite reductase (NADPH) flavoprotein alpha-component, at 724,216 to 728,973 10374 mRNA_2007: 10375 - K15331 TRMT2B, TRM2 tRNA (uracil-5-)-methyltransferase, at 729,212 to 731,398 10375 mRNA_2008: 10376 - K02929 RP-L44e, RPL44 large subunit ribosomal protein L44e, at 731,652 to 732,253 10376 mRNA_2009: 10377 - BLAST ankyrin [Cenococcum geophilum 1.58], at 733,101 to 734,076 10377
Group Condition mRNA_2005 mRNA_2006 mRNA_2007 mRNA_2008 mRNA_2009
10373 10374 10375 10376 10377
growth on ricinoleic acid YNB Glucose -0.8 -2.0 -0.5 N.D. +0.5
auxotrophy YNB Glucose -1.1 -2.2 -0.1 N.D. +0.9
auxotrophy YNB Glucose 75mM Arginine -1.0 -2.2 -0.3 N.D. +1.2
growth on oleic acid YNB Glucose -0.2 -1.6 -0.1 N.D. +0.0
facs on lipid accumulation Sorted Low BODIPY Gate +0.8 -1.6 -0.8 N.D. -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 -1.6 +0.2 N.D. -0.4
facs on lipid accumulation Sorted High BODIPY Gate -0.2 -0.4 -0.8 N.D. -0.0
facs on lipid accumulation Sorted No BODIPY Gate +0.6 -1.0 -0.9 N.D. +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid -0.9 -1.0 +0.2 N.D. +1.0
growth on oleic acid YNB Oleic Acid +0.2 -0.9 -0.0 N.D. +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 -1.1 +0.2 N.D. +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 -0.6 +0.2 N.D. +0.3
growth on ricinoleic acid YNB Ricinoleic Acid -0.6 -0.8 -0.1 N.D. +1.2
auxotrophy YNB Glucose 75mM Methionine -0.6 -0.2 +0.0 N.D. +0.7
buoyancy on lipid accumulation 40 hrs High Buoyancy -1.0 +0.9 +0.2 N.D. -0.1
auxotrophy Dropout Complete -0.6 -0.2 -0.1 N.D. +1.1
ypd buoyancy 40 hrs Median Buoyancy +0.6 -0.2 -0.1 N.D. +0.1
ypd buoyancy 40 hrs High Buoyancy +0.6 -0.2 -0.2 N.D. +0.2
growth on ypd YPD +0.1 -0.1 -0.2 N.D. +0.6
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 +0.3 +0.2 N.D. +0.1
ypd buoyancy 40 hrs Low Buoyancy +1.0 +0.5 -0.0 N.D. +0.4
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