Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10380 and 10381 are separated by 264 nucleotides10381 and 10382 are separated by 707 nucleotides10382 and 10383 overlap by 10 nucleotides10383 and 10384 are separated by 73 nucleotides mRNA_2012: 10380 - K00681 ggt gamma-glutamyltranspeptidase / glutathione hydrolase, at 740,635 to 742,993 10380 mRNA_2013: 10381 - SUPERFAMILY--SSF49899, at 743,258 to 744,708 10381 mRNA_2014: 10382 - K20697 GNBP1 gram-negative bacteria-binding protein 1, at 745,416 to 747,550 10382 mRNA_2015: 10383 - Hypothetical Protein, at 747,541 to 748,131 10383 mRNA_2016: 10384 - HMMPfam-Glycosyl transferase family group 2-PF13632,SUPERFAMILY--SSF53448, at 748,205 to 751,738 10384
Group Condition mRNA_2012 mRNA_2013 mRNA_2014 mRNA_2015 mRNA_2016
10380 10381 10382 10383 10384
facs on lipid accumulation Sorted Low BODIPY Gate -0.5 -0.1 -0.1 -0.2 -0.3
ypd buoyancy 40 hrs High Buoyancy -0.3 -0.3 -0.2 -0.0 -0.1
growth on oleic acid YNB Oleic Acid -0.5 -0.2 +0.0 -0.2 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.5 -0.2 +0.1 -0.1 +0.2
growth on oleic acid YNB Glucose -0.6 -0.2 +0.1 -0.1 +0.2
facs on lipid accumulation Sorted No BODIPY Gate -0.2 +0.0 -0.0 +0.1 -0.4
auxotrophy Dropout Complete -0.5 -0.3 +0.5 -0.1 +0.1
growth on ypd YPD -0.3 +0.1 +0.1 -0.2 +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.3 -0.4 +0.0 -0.1 -0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.1 -0.1 +0.2 -0.0 -0.2
ypd buoyancy 40 hrs Low Buoyancy +0.2 -0.3 -0.1 +0.1 -0.2
ypd buoyancy 40 hrs Median Buoyancy -0.0 -0.1 -0.1 +0.1 +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.4 +0.1 +0.0 -0.0 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 -0.2 +0.1 +0.0 +0.1
growth on ricinoleic acid YNB Glucose -0.4 -0.1 +0.3 +0.1 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.4 -0.2 -0.1 +0.1 -0.1
auxotrophy YNB Glucose -0.2 +0.0 +0.3 +0.0 +0.1
auxotrophy YNB Glucose 75mM Methionine -0.1 -0.2 +0.2 +0.2 +0.1
auxotrophy YNB Glucose 75mM Arginine -0.0 +0.1 +0.3 -0.1 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 -0.2 +0.3 +0.1 +0.3
ypd buoyancy 40 hrs No Buoyancy Separation +0.4 -0.2 +0.2 +0.4 -0.1
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