Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10383 and 10384 are separated by 73 nucleotides10384 and 10385 are separated by 756 nucleotides10385 and 10386 are separated by 703 nucleotides10386 and 10387 are separated by 280 nucleotides mRNA_2015: 10383 - Hypothetical Protein, at 747,541 to 748,131 10383 mRNA_2016: 10384 - HMMPfam-Glycosyl transferase family group 2-PF13632,SUPERFAMILY--SSF53448, at 748,205 to 751,738 10384 mRNA_2017: 10385 - K12600 SKI3, TTC37 superkiller protein 3, at 752,495 to 757,859 10385 mRNA_2018: 10386 - K09554 CDC37 cell division cycle protein 37, at 758,563 to 760,344 10386 mRNA_2019: 10387 - K07088 K07088 uncharacterized protein, at 760,625 to 762,571 10387
Group Condition mRNA_2015 mRNA_2016 mRNA_2017 mRNA_2018 mRNA_2019
10383 10384 10385 10386 10387
auxotrophy Dropout Complete -0.1 +0.1 -1.0 N.D. -0.2
auxotrophy YNB Glucose +0.0 +0.1 -0.9 N.D. -0.2
facs on lipid accumulation Sorted No BODIPY Gate +0.1 -0.4 -0.7 N.D. +0.0
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 -0.3 -0.9 N.D. +0.4
ypd buoyancy 40 hrs High Buoyancy -0.0 -0.1 -0.3 N.D. -0.4
growth on ypd YPD -0.2 +0.0 -0.8 N.D. +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 +0.2 -0.3 N.D. -0.4
facs on lipid accumulation Sorted High BODIPY Gate -0.0 -0.2 -1.3 N.D. +0.7
auxotrophy YNB Glucose 75mM Arginine -0.1 +0.1 -0.5 N.D. -0.1
growth on oleic acid YNB Glucose -0.1 +0.2 -0.4 N.D. -0.4
auxotrophy YNB Glucose 75mM Methionine +0.2 +0.1 -0.7 N.D. -0.2
growth on ricinoleic acid YNB Glucose +0.1 +0.2 -0.5 N.D. -0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 -0.1 -1.0 N.D. +0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 -0.1 -0.2 N.D. -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 +0.1 -0.1 N.D. -0.3
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 +0.3 -0.2 N.D. -0.4
growth on oleic acid YNB Oleic Acid -0.2 +0.1 +0.2 N.D. -0.0
ypd buoyancy 40 hrs Low Buoyancy +0.1 -0.2 +0.1 N.D. +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.0 +0.1 -0.1 N.D. +0.1
ypd buoyancy 40 hrs Median Buoyancy +0.1 +0.0 +0.2 N.D. -0.1
ypd buoyancy 40 hrs No Buoyancy Separation +0.4 -0.1 +0.8 N.D. +0.7
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