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Protein
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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
10387 and 10388 are separated by 146 nucleotides
10388 and 10389 are separated by 398 nucleotides
10389 and 10390 are separated by 271 nucleotides
10390 and 10391 are separated by 203 nucleotides
mRNA_2019: 10387 - K07088 K07088 uncharacterized protein, at 760,625 to 762,571
10387
mRNA_2020: 10388 - KOG1703 Adaptor protein Enigma and related PDZ-LIM proteins, at 762,718 to 765,022
10388
mRNA_2021: 10389 - BLAST deacetylase-like protein [Drechmeria coniospora], at 765,421 to 766,356
10389
mRNA_2022: 10390 - BLAST KLLA0F09339p [Kluyveromyces lactis], at 766,628 to 767,371
10390
mRNA_2023: 10391 - K04718 SPHK sphingosine kinase, at 767,575 to 770,006
10391
Group
Condition
mRNA
_2019
mRNA
_2020
mRNA
_2021
mRNA
_2022
mRNA
_2023
10387
10388
10389
10390
10391
facs on lipid accumulation
Sorted High BODIPY Gate
+0.7
-0.7
-1.7
-0.4
-0.3
auxotrophy
YNB Glucose 75mM Methionine
-0.2
+0.3
-2.0
+0.0
-0.0
auxotrophy
Dropout Complete
-0.2
+0.2
-1.0
-0.3
-0.3
growth on oleic acid
YNB Glucose
-0.4
+0.1
-0.4
-0.1
-0.5
auxotrophy
YNB Glucose
-0.2
+0.4
-1.2
-0.1
-0.2
auxotrophy
YNB Glucose 75mM Arginine
-0.1
+0.5
-1.2
-0.2
+0.1
buoyancy on lipid accumulation
40 hrs Low Buoyancy
-0.3
-0.0
-0.1
-0.1
+0.2
facs on lipid accumulation
Sorted No BODIPY Gate
+0.0
+0.2
-0.6
+0.1
+0.0
ypd buoyancy
40 hrs No Buoyancy Separation
+0.7
-0.2
-0.2
-0.1
-0.4
growth on oleic acid
YNB Oleic Acid
-0.0
+0.1
+0.4
-0.3
-0.2
buoyancy on lipid accumulation
40 hrs Median Buoyancy
+0.1
+0.1
-0.3
+0.1
+0.1
ypd buoyancy
40 hrs Median Buoyancy
-0.1
-0.1
-0.0
+0.1
+0.2
facs on lipid accumulation
Sorted Low BODIPY Gate
+0.4
+0.0
-0.4
+0.0
+0.1
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
+0.4
+0.3
-0.9
-0.2
+0.5
ypd buoyancy
40 hrs High Buoyancy
-0.4
+0.1
-0.1
+0.2
+0.4
buoyancy on lipid accumulation
40 hrs High Buoyancy
-0.1
+0.1
+0.0
+0.1
+0.1
growth on ricinoleic acid
YNB Methylricinoleic Acid
-0.4
-0.1
+0.4
+0.1
+0.3
growth on ricinoleic acid
YNB Glucose
-0.4
-0.1
+0.4
+0.3
+0.2
growth on ricinoleic acid
YNB Ricinoleic Acid
-0.4
-0.3
+0.4
+0.2
+0.5
ypd buoyancy
40 hrs Low Buoyancy
+0.1
+0.1
+0.2
+0.2
-0.1
growth on ypd
YPD
+0.1
-0.3
+0.7
+0.1
+0.2
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