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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
10389 and 10390 are separated by 271 nucleotides
10390 and 10391 are separated by 203 nucleotides
10391 and 10392 are separated by 173 nucleotides
10392 and 10393 are separated by 390 nucleotides
mRNA_2021: 10389 - BLAST deacetylase-like protein [Drechmeria coniospora], at 765,421 to 766,356
10389
mRNA_2022: 10390 - BLAST KLLA0F09339p [Kluyveromyces lactis], at 766,628 to 767,371
10390
mRNA_2023: 10391 - K04718 SPHK sphingosine kinase, at 767,575 to 770,006
10391
mRNA_2024: 10392 - K15979 SND1 staphylococcal nuclease domain-containing protein 1, at 770,180 to 773,514
10392
mRNA_2025: 10393 - SUPERFAMILY--SSF53474, at 773,905 to 776,623
10393
Group
Condition
mRNA
_2021
mRNA
_2022
mRNA
_2023
mRNA
_2024
mRNA
_2025
10389
10390
10391
10392
10393
auxotrophy
YNB Glucose 75mM Methionine
-2.0
+0.0
-0.0
-0.2
-0.3
facs on lipid accumulation
Sorted High BODIPY Gate
-1.7
-0.4
-0.3
-0.2
+0.0
auxotrophy
Dropout Complete
-1.0
-0.3
-0.3
-0.2
-0.3
auxotrophy
YNB Glucose 75mM Arginine
-1.2
-0.2
+0.1
-0.4
-0.3
auxotrophy
YNB Glucose
-1.2
-0.1
-0.2
-0.3
-0.3
growth on oleic acid
YNB Glucose
-0.4
-0.1
-0.5
-0.3
-0.2
facs on lipid accumulation
Sorted Low BODIPY Gate
-0.4
+0.0
+0.1
-0.3
-0.3
growth on oleic acid
YNB Oleic Acid
+0.4
-0.3
-0.2
-0.2
-0.3
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
-0.9
-0.2
+0.5
-0.1
+0.1
ypd buoyancy
40 hrs No Buoyancy Separation
-0.2
-0.1
-0.4
+0.2
+0.0
facs on lipid accumulation
Sorted No BODIPY Gate
-0.6
+0.1
+0.0
+0.2
-0.0
buoyancy on lipid accumulation
40 hrs Low Buoyancy
-0.1
-0.1
+0.2
-0.2
-0.1
growth on ricinoleic acid
YNB Methylricinoleic Acid
+0.4
+0.1
+0.3
-0.6
-0.4
buoyancy on lipid accumulation
40 hrs Median Buoyancy
-0.3
+0.1
+0.1
+0.0
+0.0
buoyancy on lipid accumulation
40 hrs High Buoyancy
+0.0
+0.1
+0.1
-0.2
-0.2
ypd buoyancy
40 hrs Low Buoyancy
+0.2
+0.2
-0.1
+0.2
-0.5
growth on ricinoleic acid
YNB Glucose
+0.4
+0.3
+0.2
-0.5
-0.2
ypd buoyancy
40 hrs High Buoyancy
-0.1
+0.2
+0.4
+0.2
-0.4
ypd buoyancy
40 hrs Median Buoyancy
-0.0
+0.1
+0.2
+0.3
-0.2
growth on ricinoleic acid
YNB Ricinoleic Acid
+0.4
+0.2
+0.5
-0.6
-0.1
growth on ypd
YPD
+0.7
+0.1
+0.2
-0.0
+0.2
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