Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10394 and 10395 are separated by 307 nucleotides10395 and 10396 are separated by 958 nucleotides10396 and 10397 are separated by 1135 nucleotides10397 and 10398 are separated by 288 nucleotides mRNA_2026: 10394 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF57701, at 776,938 to 779,956 10394 mRNA_2027: 10395 - K07208 RHEB Ras homolog enriched in brain, at 780,264 to 781,182 10395 mRNA_2028: 10396 - HMMPfam-TENA/THI-4/PQQC family-PF03070,SUPERFAMILY--SSF48613, at 782,141 to 783,596 10396 mRNA_2029: 10397 - K07088 K07088 uncharacterized protein, at 784,732 to 786,233 10397 mRNA_2030: 10398 - KOG2260 Cell division cycle 37 protein, CDC37, at 786,522 to 787,329 10398
Group Condition mRNA_2026 mRNA_2027 mRNA_2028 mRNA_2029 mRNA_2030
10394 10395 10396 10397 10398
facs on lipid accumulation Sorted High BODIPY Gate -0.3 N.D. -0.2 -0.4 -0.1
auxotrophy YNB Glucose 75mM Arginine -0.1 N.D. -0.3 -0.3 -0.1
auxotrophy YNB Glucose -0.1 N.D. -0.3 -0.3 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate -0.9 N.D. -0.4 +0.1 +0.4
growth on oleic acid YNB Oleic Acid -0.2 N.D. -0.2 -0.4 +0.1
auxotrophy Dropout Complete -0.1 N.D. -0.0 -0.4 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.3 N.D. -0.1 -0.1 -0.1
auxotrophy YNB Glucose 75mM Methionine +0.0 N.D. -0.3 -0.2 +0.0
ypd buoyancy 40 hrs Median Buoyancy -0.3 N.D. -0.1 +0.2 -0.0
ypd buoyancy 40 hrs High Buoyancy -0.4 N.D. -0.3 +0.4 -0.1
growth on ricinoleic acid YNB Glucose -0.0 N.D. -0.1 -0.1 -0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.1 N.D. -0.2 -0.1 +0.4
growth on oleic acid YNB Glucose -0.0 N.D. +0.0 -0.1 +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 N.D. -0.1 +0.3 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.3 N.D. +0.5 -0.0 -0.2
ypd buoyancy 40 hrs Low Buoyancy -0.3 N.D. -0.3 +0.4 +0.2
growth on ypd YPD -0.1 N.D. -0.1 -0.1 +0.4
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 N.D. +0.1 -0.0 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation -0.5 N.D. +0.1 +0.3 +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.0 N.D. +0.3 +0.0 -0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 N.D. +0.3 +0.3 +0.1
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