Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10408 and 10409 are separated by 1527 nucleotides10409 and 10410 are separated by 3120 nucleotides10410 and 10411 are separated by 924 nucleotides10411 and 10412 are separated by 480 nucleotides mRNA_2040: 10408 - K11729 ACAD10 acyl-CoA dehydrogenase family member 10, at 811,231 to 813,728 10408 mRNA_2041: 10409 - HMMPfam-SAM domain (Sterile alpha motif)-PF00536,ProSiteProfiles-SAM domain profile.-PS50105,SUPERFAMILY--SSF47769, at 815,256 to 815,459 10409 mRNA_2042: 10410 - K11228 STE11 mitogen-activated protein kinase kinase kinase, at 818,580 to 819,652 10410 mRNA_2043: 10411 - Hypothetical Protein, at 820,577 to 820,768 10411 mRNA_2044: 10412 - HMMPfam-Domain of unknown function (DUF1793)-PF08760,SUPERFAMILY--SSF48208, at 821,249 to 824,656 10412
Group Condition mRNA_2040 mRNA_2041 mRNA_2042 mRNA_2043 mRNA_2044
10408 10409 10410 10411 10412
growth on ricinoleic acid YNB Methylricinoleic Acid -2.2 N.D. N.D. N.D. +0.4
growth on ricinoleic acid YNB Ricinoleic Acid -2.3 N.D. N.D. N.D. +0.6
growth on oleic acid YNB Oleic Acid -0.7 N.D. N.D. N.D. +0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.3 N.D. N.D. N.D. -0.2
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 N.D. N.D. N.D. -0.5
ypd buoyancy 40 hrs High Buoyancy +0.0 N.D. N.D. N.D. -0.3
auxotrophy YNB Glucose 75mM Arginine -0.1 N.D. N.D. N.D. -0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.1 N.D. N.D. N.D. -0.0
auxotrophy YNB Glucose -0.2 N.D. N.D. N.D. +0.0
auxotrophy YNB Glucose 75mM Methionine -0.3 N.D. N.D. N.D. +0.1
ypd buoyancy 40 hrs Median Buoyancy +0.2 N.D. N.D. N.D. -0.3
growth on oleic acid YNB Glucose -0.1 N.D. N.D. N.D. +0.0
growth on ypd YPD -0.1 N.D. N.D. N.D. +0.1
ypd buoyancy 40 hrs Low Buoyancy -0.2 N.D. N.D. N.D. +0.2
auxotrophy Dropout Complete +0.0 N.D. N.D. N.D. +0.2
growth on ricinoleic acid YNB Glucose +0.0 N.D. N.D. N.D. +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 N.D. N.D. N.D. +0.5
ypd buoyancy 40 hrs No Buoyancy Separation +0.7 N.D. N.D. N.D. -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.0 N.D. N.D. N.D. +0.6
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.0 N.D. N.D. N.D. +0.7
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.0 N.D. N.D. N.D. +0.8
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