Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10410 and 10411 are separated by 924 nucleotides10411 and 10412 are separated by 480 nucleotides10412 and 10413 are separated by 228 nucleotides10413 and 10414 are separated by 413 nucleotides mRNA_2042: 10410 - K11228 STE11 mitogen-activated protein kinase kinase kinase, at 818,580 to 819,652 10410 mRNA_2043: 10411 - Hypothetical Protein, at 820,577 to 820,768 10411 mRNA_2044: 10412 - HMMPfam-Domain of unknown function (DUF1793)-PF08760,SUPERFAMILY--SSF48208, at 821,249 to 824,656 10412 mRNA_2045: 10413 - Hypothetical Protein, at 824,885 to 825,949 10413 mRNA_2046: 10414 - Hypothetical Protein, at 826,363 to 829,699 10414
Group Condition mRNA_2042 mRNA_2043 mRNA_2044 mRNA_2045 mRNA_2046
10410 10411 10412 10413 10414
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. -0.5 -0.9 -0.2
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. -0.3 +0.1 -0.3
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. -0.0 -0.3 +0.0
growth on oleic acid YNB Oleic Acid N.D. N.D. +0.1 -0.2 -0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. -0.2 +0.0 -0.0
auxotrophy Dropout Complete N.D. N.D. +0.2 -0.3 -0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. -0.3 +0.3 -0.1
growth on oleic acid YNB Glucose N.D. N.D. +0.0 -0.0 -0.0
growth on ricinoleic acid YNB Glucose N.D. N.D. +0.2 -0.1 +0.0
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. -0.1 +0.1 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. -0.1 +0.6 -0.2
auxotrophy YNB Glucose N.D. N.D. +0.0 +0.1 +0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. +0.2 +0.1 -0.0
growth on ypd YPD N.D. N.D. +0.1 +0.5 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. +0.4 +0.3 +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. +0.8 -0.0 -0.1
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. +0.1 +0.5 +0.3
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. +0.6 +0.2 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. +0.5 +0.4 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. +0.7 +0.2 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. +0.6 +0.3 +0.2
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