Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10421 and 10422 are separated by 194 nucleotides10422 and 10423 are separated by 146 nucleotides10423 and 10424 are separated by 47 nucleotides10424 and 10425 are separated by 379 nucleotides mRNA_2053: 10421 - KOG2450 Aldehyde dehydrogenase, at 842,019 to 844,467 10421 mRNA_2054: 10422 - KOG1994 Predicted RNA binding protein, contains G-patch and Zn-finger domains, at 844,662 to 846,096 10422 mRNA_2055: 10423 - KOG2817 Predicted E3 ubiquitin ligase, at 846,243 to 848,116 10423 mRNA_2056: 10424 - K11667 INO80C, IES6 INO80 complex subunit C, at 848,164 to 849,134 10424 mRNA_2057: 10425 - KOG3010 Methyltransferase, at 849,514 to 850,707 10425
Group Condition mRNA_2053 mRNA_2054 mRNA_2055 mRNA_2056 mRNA_2057
10421 10422 10423 10424 10425
facs on lipid accumulation Sorted High BODIPY Gate +0.1 -0.3 -2.4 -2.4 -0.3
facs on lipid accumulation Sorted No BODIPY Gate +0.0 -0.2 -0.9 -0.7 -0.4
facs on lipid accumulation Sorted Low BODIPY Gate -0.0 -0.1 -0.5 -1.3 -0.3
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 -0.3 -2.0 +0.5 -0.2
ypd buoyancy 40 hrs Median Buoyancy -0.0 -0.0 -0.2 -0.9 -0.2
ypd buoyancy 40 hrs High Buoyancy -0.1 -0.1 -0.5 -0.1 +0.4
auxotrophy YNB Glucose 75mM Arginine -0.0 +0.5 +0.3 -0.4 -0.5
auxotrophy YNB Glucose 75mM Methionine +0.1 +0.3 +0.2 -0.3 -0.4
growth on ricinoleic acid YNB Methylricinoleic Acid -0.4 -0.1 -0.5 +0.9 +0.1
growth on oleic acid YNB Glucose -0.1 +0.2 +0.1 -0.2 +0.2
growth on ricinoleic acid YNB Glucose -0.2 -0.1 -0.1 +0.4 +0.3
auxotrophy Dropout Complete +0.1 +0.3 +0.0 -0.0 -0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 +0.5 +0.4 -0.6 -0.0
growth on ypd YPD -0.1 +0.5 +0.5 -0.1 -0.4
growth on oleic acid YNB Oleic Acid -0.1 -0.3 +0.0 +1.1 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.0 +0.4 +0.0 +0.3 +0.3
ypd buoyancy 40 hrs Low Buoyancy +0.2 +0.0 +0.2 +0.6 +0.0
auxotrophy YNB Glucose +0.2 +0.4 +0.5 +0.4 -0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 +0.1 +0.4 +0.8 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.0 +0.2 +0.5 +0.8 +0.2
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 -0.3 -0.1 +2.0 +0.5
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