Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10424 and 10425 are separated by 379 nucleotides10425 and 10426 are separated by 82 nucleotides10426 and 10427 are separated by 238 nucleotides10427 and 10428 are separated by 933 nucleotides mRNA_2056: 10424 - K11667 INO80C, IES6 INO80 complex subunit C, at 848,164 to 849,134 10424 mRNA_2057: 10425 - KOG3010 Methyltransferase, at 849,514 to 850,707 10425 mRNA_2058: 10426 - KOG1128 Uncharacterized conserved protein, contains TPR repeats, at 850,790 to 853,642 10426 mRNA_2059: 10427 - K13509 AGPAT1_2 lysophosphatidate acyltransferase, at 853,881 to 854,956 10427 mRNA_2060: 10428 - K05824 LYS12 homoisocitrate dehydrogenase, at 855,890 to 857,453 10428
Group Condition mRNA_2056 mRNA_2057 mRNA_2058 mRNA_2059 mRNA_2060
10424 10425 10426 10427 10428
facs on lipid accumulation Sorted High BODIPY Gate -2.4 -0.3 N.D. N.D. -0.8
auxotrophy YNB Glucose 75mM Arginine -0.4 -0.5 N.D. N.D. -2.2
auxotrophy YNB Glucose 75mM Methionine -0.3 -0.4 N.D. N.D. -2.1
auxotrophy YNB Glucose +0.4 -0.4 N.D. N.D. -2.5
facs on lipid accumulation Sorted Low BODIPY Gate -1.3 -0.3 N.D. N.D. -0.8
facs on lipid accumulation Sorted No BODIPY Gate -0.7 -0.4 N.D. N.D. -1.3
growth on oleic acid YNB Glucose -0.2 +0.2 N.D. N.D. -2.3
growth on ricinoleic acid YNB Glucose +0.4 +0.3 N.D. N.D. -2.8
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.6 -0.0 N.D. N.D. -1.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.8 +0.1 N.D. N.D. -2.4
ypd buoyancy 40 hrs Median Buoyancy -0.9 -0.2 N.D. N.D. -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.9 +0.1 N.D. N.D. -1.8
growth on ypd YPD -0.1 -0.4 N.D. N.D. -0.2
growth on oleic acid YNB Oleic Acid +1.1 -0.2 N.D. N.D. -1.5
auxotrophy Dropout Complete -0.0 -0.0 N.D. N.D. -0.3
ypd buoyancy 40 hrs No Buoyancy Separation +0.5 -0.2 N.D. N.D. -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.8 +0.2 N.D. N.D. -0.7
ypd buoyancy 40 hrs High Buoyancy -0.1 +0.4 N.D. N.D. -0.0
growth on ricinoleic acid YNB Ricinoleic Acid +2.0 +0.5 N.D. N.D. -2.1
ypd buoyancy 40 hrs Low Buoyancy +0.6 +0.0 N.D. N.D. +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.3 +0.3 N.D. N.D. +0.7
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