Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10425 and 10426 are separated by 82 nucleotides10426 and 10427 are separated by 238 nucleotides10427 and 10428 are separated by 933 nucleotides10428 and 10429 are separated by 346 nucleotides mRNA_2057: 10425 - KOG3010 Methyltransferase, at 849,514 to 850,707 10425 mRNA_2058: 10426 - KOG1128 Uncharacterized conserved protein, contains TPR repeats, at 850,790 to 853,642 10426 mRNA_2059: 10427 - K13509 AGPAT1_2 lysophosphatidate acyltransferase, at 853,881 to 854,956 10427 mRNA_2060: 10428 - K05824 LYS12 homoisocitrate dehydrogenase, at 855,890 to 857,453 10428 mRNA_2061: 10429 - HMMPfam-Protein of unknown function (DUF1640)-PF07798, at 857,800 to 859,276 10429
Group Condition mRNA_2057 mRNA_2058 mRNA_2059 mRNA_2060 mRNA_2061
10425 10426 10427 10428 10429
auxotrophy YNB Glucose -0.4 N.D. N.D. -2.5 -0.1
growth on ricinoleic acid YNB Glucose +0.3 N.D. N.D. -2.8 -0.3
facs on lipid accumulation Sorted No BODIPY Gate -0.4 N.D. N.D. -1.3 -0.8
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 N.D. N.D. -1.8 -0.7
auxotrophy YNB Glucose 75mM Methionine -0.4 N.D. N.D. -2.1 +0.2
facs on lipid accumulation Sorted High BODIPY Gate -0.3 N.D. N.D. -0.8 -1.1
auxotrophy YNB Glucose 75mM Arginine -0.5 N.D. N.D. -2.2 +0.7
growth on oleic acid YNB Glucose +0.2 N.D. N.D. -2.3 +0.2
facs on lipid accumulation Sorted Low BODIPY Gate -0.3 N.D. N.D. -0.8 -0.6
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 N.D. N.D. -2.4 +0.7
growth on oleic acid YNB Oleic Acid -0.2 N.D. N.D. -1.5 +0.3
growth on ricinoleic acid YNB Ricinoleic Acid +0.5 N.D. N.D. -2.1 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.0 N.D. N.D. -1.3 +0.4
growth on ypd YPD -0.4 N.D. N.D. -0.2 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 N.D. N.D. -0.7 +0.1
auxotrophy Dropout Complete -0.0 N.D. N.D. -0.3 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation -0.2 N.D. N.D. -0.2 +0.5
ypd buoyancy 40 hrs Median Buoyancy -0.2 N.D. N.D. -0.1 +0.5
ypd buoyancy 40 hrs Low Buoyancy +0.0 N.D. N.D. +0.0 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.3 N.D. N.D. +0.7 -0.2
ypd buoyancy 40 hrs High Buoyancy +0.4 N.D. N.D. -0.0 +0.9
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