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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
10425 and 10426 are separated by 82 nucleotides
10426 and 10427 are separated by 238 nucleotides
10427 and 10428 are separated by 933 nucleotides
10428 and 10429 are separated by 346 nucleotides
mRNA_2057: 10425 - KOG3010 Methyltransferase, at 849,514 to 850,707
10425
mRNA_2058: 10426 - KOG1128 Uncharacterized conserved protein, contains TPR repeats, at 850,790 to 853,642
10426
mRNA_2059: 10427 - K13509 AGPAT1_2 lysophosphatidate acyltransferase, at 853,881 to 854,956
10427
mRNA_2060: 10428 - K05824 LYS12 homoisocitrate dehydrogenase, at 855,890 to 857,453
10428
mRNA_2061: 10429 - HMMPfam-Protein of unknown function (DUF1640)-PF07798, at 857,800 to 859,276
10429
Group
Condition
mRNA
_2057
mRNA
_2058
mRNA
_2059
mRNA
_2060
mRNA
_2061
10425
10426
10427
10428
10429
auxotrophy
YNB Glucose
-0.4
N.D.
N.D.
-2.5
-0.1
growth on ricinoleic acid
YNB Glucose
+0.3
N.D.
N.D.
-2.8
-0.3
facs on lipid accumulation
Sorted No BODIPY Gate
-0.4
N.D.
N.D.
-1.3
-0.8
growth on ricinoleic acid
YNB Methylricinoleic Acid
+0.1
N.D.
N.D.
-1.8
-0.7
auxotrophy
YNB Glucose 75mM Methionine
-0.4
N.D.
N.D.
-2.1
+0.2
facs on lipid accumulation
Sorted High BODIPY Gate
-0.3
N.D.
N.D.
-0.8
-1.1
auxotrophy
YNB Glucose 75mM Arginine
-0.5
N.D.
N.D.
-2.2
+0.7
growth on oleic acid
YNB Glucose
+0.2
N.D.
N.D.
-2.3
+0.2
facs on lipid accumulation
Sorted Low BODIPY Gate
-0.3
N.D.
N.D.
-0.8
-0.6
buoyancy on lipid accumulation
40 hrs Low Buoyancy
+0.1
N.D.
N.D.
-2.4
+0.7
growth on oleic acid
YNB Oleic Acid
-0.2
N.D.
N.D.
-1.5
+0.3
growth on ricinoleic acid
YNB Ricinoleic Acid
+0.5
N.D.
N.D.
-2.1
+0.1
buoyancy on lipid accumulation
40 hrs Median Buoyancy
-0.0
N.D.
N.D.
-1.3
+0.4
growth on ypd
YPD
-0.4
N.D.
N.D.
-0.2
-0.2
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
+0.2
N.D.
N.D.
-0.7
+0.1
auxotrophy
Dropout Complete
-0.0
N.D.
N.D.
-0.3
-0.1
ypd buoyancy
40 hrs No Buoyancy Separation
-0.2
N.D.
N.D.
-0.2
+0.5
ypd buoyancy
40 hrs Median Buoyancy
-0.2
N.D.
N.D.
-0.1
+0.5
ypd buoyancy
40 hrs Low Buoyancy
+0.0
N.D.
N.D.
+0.0
+0.2
buoyancy on lipid accumulation
40 hrs High Buoyancy
+0.3
N.D.
N.D.
+0.7
-0.2
ypd buoyancy
40 hrs High Buoyancy
+0.4
N.D.
N.D.
-0.0
+0.9
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