Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10427 and 10428 are separated by 933 nucleotides10428 and 10429 are separated by 346 nucleotides10429 and 10430 are separated by 763 nucleotides10430 and 10431 are separated by 399 nucleotides mRNA_2059: 10427 - K13509 AGPAT1_2 lysophosphatidate acyltransferase, at 853,881 to 854,956 10427 mRNA_2060: 10428 - K05824 LYS12 homoisocitrate dehydrogenase, at 855,890 to 857,453 10428 mRNA_2061: 10429 - HMMPfam-Protein of unknown function (DUF1640)-PF07798, at 857,800 to 859,276 10429 mRNA_2062: 10430 - K06210 NMNAT nicotinamide mononucleotide adenylyltransferase, at 860,040 to 861,424 10430 mRNA_2063: 10431 - SUPERFAMILY--SSF140860, at 861,824 to 863,143 10431
Group Condition mRNA_2059 mRNA_2060 mRNA_2061 mRNA_2062 mRNA_2063
10427 10428 10429 10430 10431
growth on ricinoleic acid YNB Glucose N.D. -2.8 -0.3 N.D. -0.7
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -1.8 -0.7 N.D. -0.8
auxotrophy YNB Glucose N.D. -2.5 -0.1 N.D. -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -2.4 +0.7 N.D. -0.4
growth on oleic acid YNB Glucose N.D. -2.3 +0.2 N.D. +0.0
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -2.1 +0.1 N.D. -0.2
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.8 -1.1 N.D. +0.2
auxotrophy YNB Glucose 75mM Methionine N.D. -2.1 +0.2 N.D. +0.2
auxotrophy YNB Glucose 75mM Arginine N.D. -2.2 +0.7 N.D. +0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. -1.3 -0.8 N.D. +1.0
growth on oleic acid YNB Oleic Acid N.D. -1.5 +0.3 N.D. +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.7 +0.1 N.D. -0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.2 +0.5 N.D. -1.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -1.3 +0.4 N.D. +0.4
auxotrophy Dropout Complete N.D. -0.3 -0.1 N.D. -0.1
growth on ypd YPD N.D. -0.2 -0.2 N.D. +0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.0 +0.2 N.D. -0.2
ypd buoyancy 40 hrs High Buoyancy N.D. -0.0 +0.9 N.D. -0.5
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.8 -0.6 N.D. +1.8
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.1 +0.5 N.D. +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.7 -0.2 N.D. +0.3
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