Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10429 and 10430 are separated by 763 nucleotides10430 and 10431 are separated by 399 nucleotides10431 and 10432 are separated by 575 nucleotides10432 and 10433 are separated by 404 nucleotides mRNA_2061: 10429 - HMMPfam-Protein of unknown function (DUF1640)-PF07798, at 857,800 to 859,276 10429 mRNA_2062: 10430 - K06210 NMNAT nicotinamide mononucleotide adenylyltransferase, at 860,040 to 861,424 10430 mRNA_2063: 10431 - SUPERFAMILY--SSF140860, at 861,824 to 863,143 10431 mRNA_2064: 10432 - KOG3839 Lectin VIP36, involved in the transport of glycoproteins carrying high mannose-type glycans, at 863,719 to 865,833 10432 mRNA_2065: 10433 - Hypothetical Protein, at 866,238 to 867,408 10433
Group Condition mRNA_2061 mRNA_2062 mRNA_2063 mRNA_2064 mRNA_2065
10429 10430 10431 10432 10433
growth on ricinoleic acid YNB Methylricinoleic Acid -0.7 N.D. -0.8 +0.2 +0.6
growth on ricinoleic acid YNB Glucose -0.3 N.D. -0.7 +0.1 +0.4
facs on lipid accumulation Sorted High BODIPY Gate -1.1 N.D. +0.2 +0.3 +0.3
auxotrophy Dropout Complete -0.1 N.D. -0.1 -0.1 +0.1
auxotrophy YNB Glucose -0.1 N.D. -0.1 -0.0 +0.2
growth on ypd YPD -0.2 N.D. +0.1 +0.2 -0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.5 N.D. -1.0 +0.0 +0.5
ypd buoyancy 40 hrs Low Buoyancy +0.2 N.D. -0.2 -0.0 +0.2
ypd buoyancy 40 hrs High Buoyancy +0.9 N.D. -0.5 -0.1 -0.1
growth on oleic acid YNB Glucose +0.2 N.D. +0.0 -0.0 +0.1
growth on oleic acid YNB Oleic Acid +0.3 N.D. +0.3 -0.0 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 N.D. -0.1 +0.1 +0.4
auxotrophy YNB Glucose 75mM Methionine +0.2 N.D. +0.2 -0.1 +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.7 N.D. -0.4 +0.1 +0.4
ypd buoyancy 40 hrs Median Buoyancy +0.5 N.D. +0.1 -0.0 +0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.8 N.D. +1.0 +0.3 +0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.2 N.D. +0.3 +0.3 +0.5
auxotrophy YNB Glucose 75mM Arginine +0.7 N.D. +0.1 -0.0 +0.2
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 N.D. -0.2 +0.4 +0.8
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.4 N.D. +0.4 +0.2 +0.5
facs on lipid accumulation Sorted Low BODIPY Gate -0.6 N.D. +1.8 +0.1 +0.1
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