Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10458 and 10459 are separated by 195 nucleotides10459 and 10460 are separated by 823 nucleotides10460 and 10461 are separated by 354 nucleotides10461 and 10462 are separated by 252 nucleotides mRNA_2090: 10458 - K09104 K09104 bHLH factor, other, at 48,252 to 49,306 10458 mRNA_2091: 10459 - KOG0254 Predicted transporter (major facilitator superfamily), at 49,502 to 51,805 10459 mRNA_2092: 10460 - K20457 DHFS dihydrofolate synthase, at 52,629 to 54,149 10460 mRNA_2093: 10461 - K10967 KTR1_3 alpha 1,2-mannosyltransferase, at 54,504 to 56,604 10461 mRNA_2094: 10462 - Hypothetical Protein, at 56,857 to 57,476 10462
Group Condition mRNA_2090 mRNA_2091 mRNA_2092 mRNA_2093 mRNA_2094
10458 10459 10460 10461 10462
facs on lipid accumulation Sorted High BODIPY Gate -1.4 +0.1 N.D. -0.1 -0.4
facs on lipid accumulation Sorted Low BODIPY Gate -1.0 -0.2 N.D. +0.0 +0.0
ypd buoyancy 40 hrs High Buoyancy -0.5 -0.1 N.D. -0.3 -0.2
growth on ricinoleic acid YNB Ricinoleic Acid -0.3 -0.2 N.D. +0.1 -0.6
ypd buoyancy 40 hrs Low Buoyancy -0.4 -0.2 N.D. -0.1 -0.2
facs on lipid accumulation Sorted No BODIPY Gate -0.3 -0.0 N.D. -0.2 -0.3
auxotrophy YNB Glucose +0.2 -0.2 N.D. -0.0 -0.7
growth on ricinoleic acid YNB Glucose -0.3 -0.2 N.D. +0.1 -0.3
ypd buoyancy 40 hrs No Buoyancy Separation -0.2 +0.4 N.D. -0.1 -0.6
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 -0.4 N.D. +0.1 -0.1
ypd buoyancy 40 hrs Median Buoyancy -0.1 -0.2 N.D. +0.0 -0.1
auxotrophy YNB Glucose 75mM Arginine +0.6 -0.3 N.D. -0.2 -0.3
auxotrophy YNB Glucose 75mM Methionine +0.5 -0.1 N.D. -0.1 -0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 -0.2 N.D. +0.1 +0.4
auxotrophy Dropout Complete +0.2 -0.2 N.D. -0.0 +0.2
growth on ypd YPD -0.1 +0.1 N.D. +0.3 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 -0.2 N.D. +0.0 +0.3
growth on oleic acid YNB Glucose +0.5 -0.2 N.D. +0.2 +0.1
growth on oleic acid YNB Oleic Acid +0.4 -0.3 N.D. +0.3 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 +0.1 N.D. +0.3 +0.5
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.9 -0.0 N.D. +0.2 +0.2
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