Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10461 and 10462 are separated by 252 nucleotides10462 and 10463 are separated by 286 nucleotides10463 and 10464 are separated by 379 nucleotides10464 and 10465 are separated by 651 nucleotides mRNA_2093: 10461 - K10967 KTR1_3 alpha 1,2-mannosyltransferase, at 54,504 to 56,604 10461 mRNA_2094: 10462 - Hypothetical Protein, at 56,857 to 57,476 10462 mRNA_2095: 10463 - Hypothetical Protein, at 57,763 to 59,527 10463 mRNA_2096: 10464 - K05754 ARPC5 actin related protein 2/3 complex, subunit 5, at 59,907 to 60,818 10464 mRNA_2097: 10465 - K03217 yidC, spoIIIJ, OXA1, ccfA YidC/Oxa1 family membrane protein insertase, at 61,470 to 63,350 10465
Group Condition mRNA_2093 mRNA_2094 mRNA_2095 mRNA_2096 mRNA_2097
10461 10462 10463 10464 10465
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 -0.6 +0.4 N.D. -2.8
facs on lipid accumulation Sorted No BODIPY Gate -0.2 -0.3 -0.1 N.D. -1.2
facs on lipid accumulation Sorted High BODIPY Gate -0.1 -0.4 -0.2 N.D. -0.7
ypd buoyancy 40 hrs Low Buoyancy -0.1 -0.2 -0.1 N.D. -0.1
growth on ricinoleic acid YNB Glucose +0.1 -0.3 -0.1 N.D. -0.2
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 -0.6 -0.2 N.D. +0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 +0.2 +0.0 N.D. -0.6
auxotrophy YNB Glucose 75mM Methionine -0.1 -0.4 +0.0 N.D. +0.4
ypd buoyancy 40 hrs High Buoyancy -0.3 -0.2 -0.1 N.D. +0.7
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 -0.1 +0.0 N.D. +0.0
ypd buoyancy 40 hrs Median Buoyancy +0.0 -0.1 -0.0 N.D. +0.1
auxotrophy YNB Glucose -0.0 -0.7 -0.1 N.D. +1.0
growth on oleic acid YNB Oleic Acid +0.3 +0.2 +0.1 N.D. -0.3
auxotrophy YNB Glucose 75mM Arginine -0.2 -0.3 -0.1 N.D. +1.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 +0.4 +0.3 N.D. -0.1
growth on oleic acid YNB Glucose +0.2 +0.1 +0.0 N.D. +0.7
facs on lipid accumulation Sorted Low BODIPY Gate +0.0 +0.0 +0.1 N.D. +1.1
auxotrophy Dropout Complete -0.0 +0.2 -0.0 N.D. +1.1
growth on ypd YPD +0.3 -0.2 +0.1 N.D. +1.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 +0.5 +0.1 N.D. +0.9
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 +0.3 +0.1 N.D. +1.9
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