Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10462 and 10463 are separated by 286 nucleotides10463 and 10464 are separated by 379 nucleotides10464 and 10465 are separated by 651 nucleotides10465 and 10466 are separated by 414 nucleotides mRNA_2094: 10462 - Hypothetical Protein, at 56,857 to 57,476 10462 mRNA_2095: 10463 - Hypothetical Protein, at 57,763 to 59,527 10463 mRNA_2096: 10464 - K05754 ARPC5 actin related protein 2/3 complex, subunit 5, at 59,907 to 60,818 10464 mRNA_2097: 10465 - K03217 yidC, spoIIIJ, OXA1, ccfA YidC/Oxa1 family membrane protein insertase, at 61,470 to 63,350 10465 mRNA_2098: 10466 - Hypothetical Protein, at 63,765 to 64,899 10466
Group Condition mRNA_2094 mRNA_2095 mRNA_2096 mRNA_2097 mRNA_2098
10462 10463 10464 10465 10466
ypd buoyancy 40 hrs No Buoyancy Separation -0.6 +0.4 N.D. -2.8 -0.4
facs on lipid accumulation Sorted No BODIPY Gate -0.3 -0.1 N.D. -1.2 +0.4
facs on lipid accumulation Sorted High BODIPY Gate -0.4 -0.2 N.D. -0.7 +0.3
ypd buoyancy 40 hrs Low Buoyancy -0.2 -0.1 N.D. -0.1 -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 +0.0 N.D. -0.6 -0.1
ypd buoyancy 40 hrs Median Buoyancy -0.1 -0.0 N.D. +0.1 -0.2
growth on ricinoleic acid YNB Glucose -0.3 -0.1 N.D. -0.2 +0.6
ypd buoyancy 40 hrs High Buoyancy -0.2 -0.1 N.D. +0.7 -0.2
growth on ricinoleic acid YNB Ricinoleic Acid -0.6 -0.2 N.D. +0.4 +0.6
auxotrophy YNB Glucose 75mM Methionine -0.4 +0.0 N.D. +0.4 +0.2
auxotrophy YNB Glucose -0.7 -0.1 N.D. +1.0 +0.4
growth on oleic acid YNB Oleic Acid +0.2 +0.1 N.D. -0.3 +0.6
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.4 +0.3 N.D. -0.1 -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 +0.0 N.D. +0.0 +0.8
growth on ypd YPD -0.2 +0.1 N.D. +1.1 -0.2
auxotrophy YNB Glucose 75mM Arginine -0.3 -0.1 N.D. +1.2 +0.3
growth on oleic acid YNB Glucose +0.1 +0.0 N.D. +0.7 +0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.5 +0.1 N.D. +0.9 -0.0
facs on lipid accumulation Sorted Low BODIPY Gate +0.0 +0.1 N.D. +1.1 +0.4
auxotrophy Dropout Complete +0.2 -0.0 N.D. +1.1 +0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.3 +0.1 N.D. +1.9 +0.4
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