Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10468 and 10469 are separated by 179 nucleotides10469 and 10470 are separated by 324 nucleotides10470 and 10471 are separated by 168 nucleotides10471 and 10472 are separated by 52 nucleotides mRNA_2100: 10468 - K13184 DHX9 ATP-dependent RNA helicase A, at 68,093 to 72,993 10468 mRNA_2101: 10469 - K12041 SLC9A6_7, NHE6_7 solute carrier family 9 (sodium/hydrogen exchanger), member 6/7, at 73,173 to 76,460 10469 mRNA_2102: 10470 - SUPERFAMILY--SSF53067, at 76,785 to 80,345 10470 mRNA_2103: 10471 - Hypothetical Protein, at 80,514 to 81,311 10471 mRNA_2104: 10472 - KOG0101 Molecular chaperones HSP70/HSC70, HSP70 superfamily, at 81,364 to 84,462 10472
Group Condition mRNA_2100 mRNA_2101 mRNA_2102 mRNA_2103 mRNA_2104
10468 10469 10470 10471 10472
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -1.3 -0.1 +0.1 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.8 +0.1 +0.3 -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.7 +0.1 +0.0 -0.0
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.7 +0.1 -0.0 -0.0
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.7 +0.0 +0.1 +0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.4 +0.1 -0.1 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.2 +0.1 -0.1 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.4 +0.3 -0.1 +0.3
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.0 +0.1 +0.1 -0.1
auxotrophy YNB Glucose 75mM Arginine N.D. +0.3 -0.1 +0.1 -0.1
growth on ypd YPD N.D. -0.2 +0.2 +0.3 +0.1
ypd buoyancy 40 hrs High Buoyancy N.D. +0.0 +0.0 +0.2 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.4 +0.1 -0.1 +0.0
auxotrophy Dropout Complete N.D. +0.3 -0.0 +0.3 +0.1
auxotrophy YNB Glucose 75mM Methionine N.D. +0.3 +0.1 +0.4 +0.0
growth on oleic acid YNB Glucose N.D. +0.7 +0.1 -0.0 +0.2
auxotrophy YNB Glucose N.D. +0.6 +0.2 +0.2 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +1.0 -0.1 +0.3 +0.0
growth on oleic acid YNB Oleic Acid N.D. +0.8 -0.0 +0.1 +0.3
growth on ricinoleic acid YNB Glucose N.D. +0.9 +0.2 +0.0 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +1.4 -0.1 +0.1 +0.1
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