Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10471 and 10472 are separated by 52 nucleotides10472 and 10473 are separated by 579 nucleotides10473 and 10474 are separated by 326 nucleotides10474 and 10475 are separated by 705 nucleotides mRNA_2103: 10471 - Hypothetical Protein, at 80,514 to 81,311 10471 mRNA_2104: 10472 - KOG0101 Molecular chaperones HSP70/HSC70, HSP70 superfamily, at 81,364 to 84,462 10472 mRNA_2105: 10473 - K17991 PXG peroxygenase, at 85,042 to 86,447 10473 mRNA_2106: 10474 - K15710 SHPRH E3 ubiquitin-protein ligase SHPRH, at 86,774 to 93,150 10474 mRNA_2107: 10475 - K01918 panC pantoate--beta-alanine ligase, at 93,856 to 95,198 10475
Group Condition mRNA_2103 mRNA_2104 mRNA_2105 mRNA_2106 mRNA_2107
10471 10472 10473 10474 10475
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 +0.0 -0.4 +0.0 -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 +0.1 -0.2 +0.1 -0.3
auxotrophy YNB Glucose 75mM Arginine +0.1 -0.1 -0.0 -0.1 -0.1
growth on ricinoleic acid YNB Glucose +0.0 +0.2 +0.1 +0.0 -0.4
auxotrophy YNB Glucose 75mM Methionine +0.4 +0.0 -0.4 +0.0 -0.1
ypd buoyancy 40 hrs Low Buoyancy +0.1 -0.1 +0.2 -0.1 -0.0
auxotrophy Dropout Complete +0.3 +0.1 -0.3 +0.1 -0.2
auxotrophy YNB Glucose +0.2 +0.1 +0.1 +0.0 -0.2
ypd buoyancy 40 hrs Median Buoyancy -0.1 +0.1 +0.0 +0.0 +0.0
growth on ypd YPD +0.3 +0.1 -0.3 +0.1 +0.1
ypd buoyancy 40 hrs High Buoyancy +0.2 +0.1 +0.1 +0.1 -0.2
growth on oleic acid YNB Oleic Acid +0.1 +0.3 -0.1 +0.2 +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 +0.0 +0.2 -0.0 +0.4
growth on oleic acid YNB Glucose -0.0 +0.2 +0.1 +0.1 +0.2
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 -0.1 +0.3 +0.1 +0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.1 +0.1 +0.3 +0.1 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.0 -0.0 +0.5 +0.1 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 -0.3 +0.6 -0.1 +0.3
facs on lipid accumulation Sorted High BODIPY Gate -0.0 -0.0 +0.6 +0.1 +0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 +0.3 +0.3 +0.2 +0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 +0.1 +0.5 +0.2 +0.4
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