Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10489 and 10490 are separated by 1130 nucleotides10490 and 10491 are separated by 348 nucleotides10491 and 10492 are separated by 60 nucleotides10492 and 10493 are separated by 391 nucleotides mRNA_2121: 10489 - K17991 PXG peroxygenase, at 121,585 to 123,428 10489 mRNA_2122: 10490 - K00103 GULO L-gulonolactone oxidase, at 124,559 to 126,866 10490 mRNA_2123: 10491 - HMMPfam-Rare lipoprotein A (RlpA)-like double-psi beta-barrel-PF03330,SUPERFAMILY--SSF50685, at 127,215 to 128,574 10491 mRNA_2124: 10492 - Hypothetical Protein, at 128,635 to 129,596 10492 mRNA_2125: 10493 - BLAST BA75_02480T0 [Komagataella pastoris], at 129,988 to 130,716 10493
Group Condition mRNA_2121 mRNA_2122 mRNA_2123 mRNA_2124 mRNA_2125
10489 10490 10491 10492 10493
growth on ricinoleic acid YNB Methylricinoleic Acid -0.3 -0.8 -0.3 +0.5 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.4 -0.3 +0.1 -0.3 -0.0
auxotrophy YNB Glucose 75mM Methionine -0.2 +0.3 -0.2 +0.1 -0.9
ypd buoyancy 40 hrs No Buoyancy Separation -0.2 -0.7 -0.3 -0.1 +0.6
growth on oleic acid YNB Glucose -0.3 -0.1 +0.2 +0.0 -0.4
growth on oleic acid YNB Oleic Acid -0.4 +0.2 -0.5 +0.2 +0.0
ypd buoyancy 40 hrs Median Buoyancy +0.1 -0.1 -0.4 -0.3 +0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 -0.6 +0.3 +0.2 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 +0.1 +0.3 -0.2 -0.2
auxotrophy YNB Glucose 75mM Arginine -0.3 +0.5 +0.1 +0.3 -0.7
facs on lipid accumulation Sorted High BODIPY Gate -0.1 -0.4 +0.4 +0.1 -0.1
auxotrophy Dropout Complete +0.2 +0.4 -0.5 +0.3 -0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 -0.1 +0.4 +0.1 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 -0.2 +0.2 +0.2 +0.0
auxotrophy YNB Glucose -0.1 +0.5 +0.1 +0.3 -0.5
ypd buoyancy 40 hrs Low Buoyancy +0.2 -0.1 +0.1 -0.1 +0.3
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 +0.2 -0.4 +0.7 +0.2
facs on lipid accumulation Sorted No BODIPY Gate +0.0 -0.1 +0.1 +0.3 +0.1
growth on ricinoleic acid YNB Glucose -0.3 +0.1 +0.2 +0.5 +0.1
ypd buoyancy 40 hrs High Buoyancy +0.3 -0.2 +0.2 -0.1 +0.7
growth on ypd YPD -0.1 +0.0 +0.9 +0.4 +0.1
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