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Protein
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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
10490 and 10491 are separated by 348 nucleotides
10491 and 10492 are separated by 60 nucleotides
10492 and 10493 are separated by 391 nucleotides
10493 and 10494 are separated by 226 nucleotides
mRNA_2122: 10490 - K00103 GULO L-gulonolactone oxidase, at 124,559 to 126,866
10490
mRNA_2123: 10491 - HMMPfam-Rare lipoprotein A (RlpA)-like double-psi beta-barrel-PF03330,SUPERFAMILY--SSF50685, at 127,215 to 128,574
10491
mRNA_2124: 10492 - Hypothetical Protein, at 128,635 to 129,596
10492
mRNA_2125: 10493 - BLAST BA75_02480T0 [Komagataella pastoris], at 129,988 to 130,716
10493
mRNA_2126: 10494 - Hypothetical Protein, at 130,943 to 133,180
10494
Group
Condition
mRNA
_2122
mRNA
_2123
mRNA
_2124
mRNA
_2125
mRNA
_2126
10490
10491
10492
10493
10494
auxotrophy
YNB Glucose 75mM Methionine
+0.3
-0.2
+0.1
-0.9
-0.3
ypd buoyancy
40 hrs No Buoyancy Separation
-0.7
-0.3
-0.1
+0.6
-0.3
ypd buoyancy
40 hrs Median Buoyancy
-0.1
-0.4
-0.3
+0.4
-0.3
growth on ricinoleic acid
YNB Methylricinoleic Acid
-0.8
-0.3
+0.5
-0.2
+0.0
buoyancy on lipid accumulation
40 hrs High Buoyancy
-0.3
+0.1
-0.3
-0.0
-0.0
auxotrophy
Dropout Complete
+0.4
-0.5
+0.3
-0.4
-0.3
buoyancy on lipid accumulation
40 hrs Median Buoyancy
-0.6
+0.3
+0.2
-0.1
-0.3
growth on oleic acid
YNB Glucose
-0.1
+0.2
+0.0
-0.4
-0.1
buoyancy on lipid accumulation
40 hrs Low Buoyancy
+0.1
+0.3
-0.2
-0.2
-0.2
auxotrophy
YNB Glucose 75mM Arginine
+0.5
+0.1
+0.3
-0.7
-0.1
growth on oleic acid
YNB Oleic Acid
+0.2
-0.5
+0.2
+0.0
+0.3
auxotrophy
YNB Glucose
+0.5
+0.1
+0.3
-0.5
-0.1
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
-0.1
+0.4
+0.1
-0.1
-0.0
growth on ricinoleic acid
YNB Ricinoleic Acid
+0.2
-0.4
+0.7
+0.2
-0.2
ypd buoyancy
40 hrs Low Buoyancy
-0.1
+0.1
-0.1
+0.3
+0.2
facs on lipid accumulation
Sorted High BODIPY Gate
-0.4
+0.4
+0.1
-0.1
+0.4
growth on ricinoleic acid
YNB Glucose
+0.1
+0.2
+0.5
+0.1
-0.2
ypd buoyancy
40 hrs High Buoyancy
-0.2
+0.2
-0.1
+0.7
+0.3
facs on lipid accumulation
Sorted Low BODIPY Gate
-0.2
+0.2
+0.2
+0.0
+0.8
facs on lipid accumulation
Sorted No BODIPY Gate
-0.1
+0.1
+0.3
+0.1
+0.8
growth on ypd
YPD
+0.0
+0.9
+0.4
+0.1
+0.0
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