Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10498 and 10499 are separated by 1670 nucleotides10499 and 10500 are separated by 406 nucleotides10500 and 10501 are separated by 456 nucleotides10501 and 10502 are separated by 326 nucleotides mRNA_2130: 10498 - Hypothetical Protein, at 138,636 to 139,652 10498 mRNA_2131: 10499 - K09580 PDIA1, P4HB protein disulfide-isomerase A1, at 141,323 to 143,619 10499 mRNA_2132: 10500 - Hypothetical Protein, at 144,026 to 145,888 10500 mRNA_2133: 10501 - ProSitePatterns-Zinc finger RanBP2-type signature.-PS01358,ProSiteProfiles-Zinc finger RanBP2 type profile.-PS50199,SMART-Zinc finger domain-SM00547,SUPERFAMILY--SSF90209, at 146,345 to 148,532 10501 mRNA_2134: 10502 - K10660 MARCH5 E3 ubiquitin-protein ligase MARCH5, at 148,859 to 150,709 10502
Group Condition mRNA_2130 mRNA_2131 mRNA_2132 mRNA_2133 mRNA_2134
10498 10499 10500 10501 10502
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 -3.6 -0.8 -0.2 +0.2
facs on lipid accumulation Sorted No BODIPY Gate -0.3 -2.2 -0.3 +0.3 +0.2
ypd buoyancy 40 hrs High Buoyancy -0.2 -0.3 -0.1 -0.8 -0.6
facs on lipid accumulation Sorted High BODIPY Gate -0.5 -1.5 +0.0 +0.4 +0.6
ypd buoyancy 40 hrs Median Buoyancy +0.1 -0.0 +0.2 -0.5 -0.2
growth on ypd YPD -0.3 +0.2 -0.1 +0.2 +0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 +1.5 +0.0 -1.6 +0.3
growth on oleic acid YNB Glucose -0.1 +0.3 -0.1 +0.7 -0.7
ypd buoyancy 40 hrs No Buoyancy Separation -0.3 +0.2 +0.6 -0.2 -0.2
auxotrophy YNB Glucose 75mM Methionine +0.5 +0.5 -0.1 -0.6 +0.1
auxotrophy YNB Glucose 75mM Arginine +0.6 +0.7 -0.3 -0.4 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.3 +0.9 -0.5 +0.2 +0.4
auxotrophy Dropout Complete +0.5 +0.8 -0.5 -0.1 +0.2
growth on oleic acid YNB Oleic Acid +0.3 +0.9 -0.3 +0.5 -0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.6 +0.5 -0.8 +0.4 +0.3
auxotrophy YNB Glucose -0.1 +1.2 -0.0 -0.3 +0.5
growth on ricinoleic acid YNB Glucose +0.4 +0.8 +0.0 +0.4 -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 +1.7 -0.7 +0.6 +0.1
ypd buoyancy 40 hrs Low Buoyancy +0.3 +1.6 +0.3 -0.0 -0.4
growth on ricinoleic acid YNB Methylricinoleic Acid +0.9 +1.9 -0.1 +0.6 -0.4
growth on ricinoleic acid YNB Ricinoleic Acid +0.7 +2.1 +0.3 +0.5 -0.4
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