Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10499 and 10500 are separated by 406 nucleotides10500 and 10501 are separated by 456 nucleotides10501 and 10502 are separated by 326 nucleotides10502 and 10503 are separated by 131 nucleotides mRNA_2131: 10499 - K09580 PDIA1, P4HB protein disulfide-isomerase A1, at 141,323 to 143,619 10499 mRNA_2132: 10500 - Hypothetical Protein, at 144,026 to 145,888 10500 mRNA_2133: 10501 - ProSitePatterns-Zinc finger RanBP2-type signature.-PS01358,ProSiteProfiles-Zinc finger RanBP2 type profile.-PS50199,SMART-Zinc finger domain-SM00547,SUPERFAMILY--SSF90209, at 146,345 to 148,532 10501 mRNA_2134: 10502 - K10660 MARCH5 E3 ubiquitin-protein ligase MARCH5, at 148,859 to 150,709 10502 mRNA_2135: 10503 - KOG2922 Uncharacterized conserved protein, at 150,841 to 152,742 10503
Group Condition mRNA_2131 mRNA_2132 mRNA_2133 mRNA_2134 mRNA_2135
10499 10500 10501 10502 10503
facs on lipid accumulation Sorted Low BODIPY Gate -3.6 -0.8 -0.2 +0.2 -0.0
facs on lipid accumulation Sorted No BODIPY Gate -2.2 -0.3 +0.3 +0.2 +0.1
ypd buoyancy 40 hrs High Buoyancy -0.3 -0.1 -0.8 -0.6 +0.0
ypd buoyancy 40 hrs Median Buoyancy -0.0 +0.2 -0.5 -0.2 +0.1
facs on lipid accumulation Sorted High BODIPY Gate -1.5 +0.0 +0.4 +0.6 +0.3
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 +0.6 -0.2 -0.2 -0.4
auxotrophy YNB Glucose 75mM Arginine +0.7 -0.3 -0.4 -0.1 +0.1
auxotrophy YNB Glucose 75mM Methionine +0.5 -0.1 -0.6 +0.1 +0.3
growth on oleic acid YNB Glucose +0.3 -0.1 +0.7 -0.7 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +1.5 +0.0 -1.6 +0.3 +0.2
auxotrophy Dropout Complete +0.8 -0.5 -0.1 +0.2 +0.1
growth on ypd YPD +0.2 -0.1 +0.2 +0.0 +0.3
growth on oleic acid YNB Oleic Acid +0.9 -0.3 +0.5 -0.4 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.5 -0.8 +0.4 +0.3 +0.4
growth on ricinoleic acid YNB Glucose +0.8 +0.0 +0.4 -0.3 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.9 -0.5 +0.2 +0.4 +0.4
ypd buoyancy 40 hrs Low Buoyancy +1.6 +0.3 -0.0 -0.4 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +1.7 -0.7 +0.6 +0.1 +0.1
auxotrophy YNB Glucose +1.2 -0.0 -0.3 +0.5 +0.4
growth on ricinoleic acid YNB Methylricinoleic Acid +1.9 -0.1 +0.6 -0.4 +0.2
growth on ricinoleic acid YNB Ricinoleic Acid +2.1 +0.3 +0.5 -0.4 +0.1
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