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Protein
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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
10500 and 10501 are separated by 456 nucleotides
10501 and 10502 are separated by 326 nucleotides
10502 and 10503 are separated by 131 nucleotides
10503 and 10504 are separated by 287 nucleotides
mRNA_2132: 10500 - Hypothetical Protein, at 144,026 to 145,888
10500
mRNA_2133: 10501 - ProSitePatterns-Zinc finger RanBP2-type signature.-PS01358,ProSiteProfiles-Zinc finger RanBP2 type profile.-PS50199,SMART-Zinc finger domain-SM00547,SUPERFAMILY--SSF90209, at 146,345 to 148,532
10501
mRNA_2134: 10502 - K10660 MARCH5 E3 ubiquitin-protein ligase MARCH5, at 148,859 to 150,709
10502
mRNA_2135: 10503 - KOG2922 Uncharacterized conserved protein, at 150,841 to 152,742
10503
mRNA_2136: 10504 - K01613 psd, PISD phosphatidylserine decarboxylase, at 153,030 to 154,707
10504
Group
Condition
mRNA
_2132
mRNA
_2133
mRNA
_2134
mRNA
_2135
mRNA
_2136
10500
10501
10502
10503
10504
ypd buoyancy
40 hrs High Buoyancy
-0.1
-0.8
-0.6
+0.0
+0.1
auxotrophy
YNB Glucose 75mM Arginine
-0.3
-0.4
-0.1
+0.1
-0.3
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
+0.0
-1.6
+0.3
+0.2
+0.0
facs on lipid accumulation
Sorted Low BODIPY Gate
-0.8
-0.2
+0.2
-0.0
+0.1
auxotrophy
YNB Glucose 75mM Methionine
-0.1
-0.6
+0.1
+0.3
-0.2
ypd buoyancy
40 hrs Median Buoyancy
+0.2
-0.5
-0.2
+0.1
+0.0
auxotrophy
Dropout Complete
-0.5
-0.1
+0.2
+0.1
-0.1
ypd buoyancy
40 hrs No Buoyancy Separation
+0.6
-0.2
-0.2
-0.4
-0.1
growth on ricinoleic acid
YNB Glucose
+0.0
+0.4
-0.3
+0.1
-0.5
growth on oleic acid
YNB Glucose
-0.1
+0.7
-0.7
+0.2
-0.0
ypd buoyancy
40 hrs Low Buoyancy
+0.3
-0.0
-0.4
+0.1
-0.0
facs on lipid accumulation
Sorted No BODIPY Gate
-0.3
+0.3
+0.2
+0.1
-0.2
growth on oleic acid
YNB Oleic Acid
-0.3
+0.5
-0.4
+0.2
+0.2
buoyancy on lipid accumulation
40 hrs High Buoyancy
-0.7
+0.6
+0.1
+0.1
+0.1
growth on ypd
YPD
-0.1
+0.2
+0.0
+0.3
-0.2
auxotrophy
YNB Glucose
-0.0
-0.3
+0.5
+0.4
-0.3
growth on ricinoleic acid
YNB Methylricinoleic Acid
-0.1
+0.6
-0.4
+0.2
-0.0
buoyancy on lipid accumulation
40 hrs Low Buoyancy
-0.8
+0.4
+0.3
+0.4
+0.0
buoyancy on lipid accumulation
40 hrs Median Buoyancy
-0.5
+0.2
+0.4
+0.4
-0.0
growth on ricinoleic acid
YNB Ricinoleic Acid
+0.3
+0.5
-0.4
+0.1
-0.1
facs on lipid accumulation
Sorted High BODIPY Gate
+0.0
+0.4
+0.6
+0.3
-0.1
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