Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10506 and 10507 are separated by 275 nucleotides10507 and 10508 are separated by 441 nucleotides10508 and 10509 are separated by 931 nucleotides10509 and 10510 are separated by 1519 nucleotides mRNA_2138: 10506 - HMMPfam-Putative stress-responsive nuclear envelope protein-PF10281, at 157,835 to 161,101 10506 mRNA_2139: 10507 - K09377 CSRP cysteine and glycine-rich protein, at 161,377 to 162,483 10507 mRNA_2140: 10508 - HMMPfam-F-box domain-PF00646,HMMPfam-Domain of unknown function (DUF1708)-PF08101,ProSiteProfiles-F-box domain profile.-PS50181,SMART-A Receptor for Ubiquitination Targets-SM00256,SUPERFAMILY--SSF48350,SUPERFAMILY--SSF81383, at 162,925 to 174,925 10508 mRNA_2141: 10509 - SUPERFAMILY--SSF52047, at 175,857 to 177,524 10509 mRNA_2142: 10510 - Hypothetical Protein, at 179,044 to 180,564 10510
Group Condition mRNA_2138 mRNA_2139 mRNA_2140 mRNA_2141 mRNA_2142
10506 10507 10508 10509 10510
growth on oleic acid YNB Glucose -0.1 -0.1 -0.1 -0.2 -0.3
facs on lipid accumulation Sorted High BODIPY Gate -0.3 -0.3 +0.1 +0.1 -0.4
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 -0.5 -0.0 -0.2 +0.1
auxotrophy YNB Glucose 75mM Arginine -0.0 -0.4 -0.1 -0.2 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.0 -0.2 -0.2 -0.1 -0.2
auxotrophy Dropout Complete -0.3 -0.6 -0.0 +0.2 +0.1
ypd buoyancy 40 hrs Low Buoyancy +0.3 -0.5 -0.1 +0.0 -0.3
growth on ricinoleic acid YNB Glucose +0.0 -0.6 -0.1 -0.1 +0.2
growth on oleic acid YNB Oleic Acid -0.1 -0.0 -0.1 -0.3 -0.1
auxotrophy YNB Glucose 75mM Methionine -0.0 -0.3 -0.1 -0.0 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.2 -0.1 -0.0 +0.2 -0.2
facs on lipid accumulation Sorted Low BODIPY Gate -0.3 -0.2 +0.0 -0.0 +0.2
auxotrophy YNB Glucose -0.0 -0.5 -0.0 +0.3 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 -0.2 +0.1 +0.4 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 -0.1 -0.0 +0.4 -0.4
ypd buoyancy 40 hrs Median Buoyancy +0.1 +0.4 -0.0 -0.0 -0.1
ypd buoyancy 40 hrs High Buoyancy +0.0 +0.0 -0.1 +0.1 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 -0.3 +0.1 +0.8 -0.2
facs on lipid accumulation Sorted No BODIPY Gate +0.1 +0.4 +0.1 -0.0 +0.1
growth on ypd YPD -0.0 +0.1 +0.1 +0.7 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation -0.2 +0.7 -0.0 +0.3 +0.3
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